EFHD2 Antibody / EF-hand domain-containing protein D2 Antibody (FY13590)

EFHD2 Antibody by IHC in mouse thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse thymus using EF-hand domain-containing protein D2 Antibody shows brown staining among cells in the thymic tissue, contrasting with hematoxylin-stained nuclei. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen generated brown immunoreactivity, with hematoxylin counterstaining highlighting nuclei.

EFHD2 Antibody by WB in human cell lines and mouse spleen. Western blot analysis using EF-hand domain-containing protein D2 Antibody detects a prominent band near 27 kDa, compared with the calculated molecular weight of approximately 27 kDa. Lanes 1-4 contain A431, A549, THP-1 and PC-3 whole-cell lysates; lane 5 contains mouse spleen lysate. Reduced lysates containing 30 ug total protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection employed an HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate.

EFHD2 Antibody by IHC in human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil using EF-hand domain-containing protein D2 Antibody shows brown staining in portions of the lymphoid tissue, with heterogeneous signal across the section. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen generated brown immunoreactivity, with hematoxylin counterstaining highlighting nuclei.

EFHD2 Antibody by IHC in rat thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded rat thymus using EF-hand domain-containing protein D2 Antibody shows brown immunoreactivity within thymic tissue against a blue nuclear counterstain. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen generated brown immunoreactivity, with hematoxylin counterstaining highlighting nuclei.

EFHD2 Antibody by IP in A549 cells. Immunoprecipitation of EF-hand domain-containing protein D2 Antibody-associated material from A549 whole-cell lysate was assessed by Western blot. The input lysate is shown in lane 1, a rabbit IgG control immunoprecipitate in lane 2, and the specific antibody immunoprecipitate in lane 3. A band near 27 kDa is visible in the input and antibody-enriched material, consistent with the expected EFHD2 molecular weight. The approximately 50 kDa signal in immunoprecipitation lanes corresponds to immunoglobulin heavy chain. The assay used 2 ug immunoprecipitating antibody and 500 ug lysate.