EFHD1 Antibody / EF-hand domain-containing protein D1 Antibody (FY13648)

EFHD1 Antibody by IHC in human breast cancer tissue. Immunohistochemistry of paraffin-embedded human breast cancer tissue using EF-hand domain-containing protein D1 Antibody. Brown staining is evident within malignant epithelial structures, with blue nuclear counterstaining. Antigen retrieval was performed with EDTA buffer at pH 8.0, followed by 10% goat serum blocking. The primary antibody was incubated at 2 ug/ml overnight at 4oC, and signal was developed with a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB.

EFHD1 Antibody by IHC in rat kidney tissue. Immunohistochemistry of paraffin-embedded rat kidney tissue using EF-hand domain-containing protein D1 Antibody. Brown DAB staining is evident in renal tubular structures, while the densely packed nuclei of a glomerular profile show blue counterstaining. The section underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocking with 10% goat serum. Primary antibody was applied at 2 ug/ml overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection.

EFHD1 Antibody by IF in MCF-7 cells. Immunofluorescence of MCF-7 cells using EF-hand domain-containing protein D1 Antibody shows green extranuclear immunoreactivity surrounding DAPI-stained blue nuclei. Cells underwent enzyme-mediated antigen retrieval for 15 minutes, were blocked with 10% goat serum and incubated with 5 ug/ml primary antibody overnight at 4oC. Fluoro488-conjugated goat anti-rabbit IgG was used at 1:500, followed by DAPI nuclear counterstaining. The image demonstrates a predominantly cytoplasmic staining pattern without identifying a specific organelle.

EFHD1 Antibody by WB in MCF-7 cells. Western blot analysis using EF-hand domain-containing protein D1 Antibody on MCF-7 whole-cell lysate resolved by 12% SDS-PAGE. A prominent immunoreactive band appears near 27 kDa, consistent with the calculated EFHD1 molecular weight of approximately 26.9 kDa. Additional weaker bands are visible at higher molecular weights, but their identities are not established by this experiment. Detection used a rabbit primary antibody followed by an HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate.