EEF1E1 Antibody / Eukaryotic translation elongation factor 1 epsilon-1 Antibody (FY13610)

EEF1E1 Antibody by IHC in human endometrial carcinoma. Immunohistochemistry of paraffin-embedded human endometrial carcinoma using Eukaryotic translation elongation factor 1 epsilon-1 Antibody shows widespread brown cytoplasmic staining in malignant glandular epithelium. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Immunoreactivity was visualized with an HRP-conjugated secondary antibody and DAB chromogen; hematoxylin counterstaining identifies tissue nuclei.

EEF1E1 Antibody by WB in human and rodent samples. Western blot analysis using Eukaryotic translation elongation factor 1 epsilon-1 Antibody detects a band near 16 kDa in human 293T (lane 1), HeLa (lane 2), Jurkat (lane 3), HepG2 (lane 4), rat testis (lane 5), and mouse testis (lane 6). This band migrates below the calculated molecular weight of approximately 20 kDa. Reduced lysates containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and chemiluminescence.

EEF1E1 Antibody by IHC in rat brain. Immunohistochemistry of paraffin-embedded rat brain using Eukaryotic translation elongation factor 1 epsilon-1 Antibody shows brown staining in scattered neuronal cell bodies and neuropil. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Immunoreactivity was visualized with an HRP-conjugated secondary antibody and DAB chromogen; hematoxylin counterstaining identifies tissue nuclei.