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Home >> Antibodies >> EEF1A1/2 Antibody

EEF1A1/2 Antibody (F54864)

  Catalog No Formulation Size Price (USD)  
Image F54864-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54864-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 293T cell lysate with EEF1A1/2 antibody and a GADPH loading control Ab. Predicted molecular weight ~50 kDa.
Western blot testing of human 1) Y79 and 2) T-47D cell lysate with EEF1A1/2 antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human brain tissue with EEF1A1/2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human NCI-H292 cells with EEF1A1/2 antibody; Blue=isotype control, Green= EEF1A1/2 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P68104
Localization Cytoplasmic, nuclear
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Western blot : 1:500-1:1000
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This EEF1A1/2 antibody is available for research use only.
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  • Applications : FACS, IF, IHC-P, WB
    Reactivity : Human

Description

EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.

Application Notes

The stated application concentrations are suggested starting points. Titration of the EEF1A1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 46-72 from the human EEF1A1 protein was used as the immunogen for the EEF1A1/2 antibody.

Storage

Aliquot the EEF1A1/2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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