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Home >> Antibodies >> EEA1 Antibody

EEA1 Antibody (RQ4105)

  Catalog No Formulation Size Price (USD)  
Image RQ4105 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO320, 4) 22RV1 and 5) A549 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.
Western blot testing of rat 1) spleen, 2) thymus, 3) brain, 4) lung and mouse 5) spleen, 6) brain, 7) HEPA1-6 and 8) NIH3T3 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q15075
Applications Western Blot : 0.5-1ug/ml
Limitations This EEA1 antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human

Description

The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo.

Application Notes

Optimal dilution of the EEA1 antibody should be determined by the researcher.

Immunogen

Amino acids RENQSLQIKHTQALNRKWAEDNEVQN from the human protein were used as the immunogen for the EEA1 antibody.

Storage

After reconstitution, the EEA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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