ECH1 Antibody / Enoyl-CoA hydratase 1 Antibody (FY13600)

ECH1 Antibody by IF in SiHa cells. Immunofluorescence using Enoyl-CoA hydratase 1 Antibody shows punctate green cytoplasmic staining concentrated around the nuclei of SiHa cells. Red alpha-tubulin labeling delineates the cytoskeleton, and DAPI stains the nuclei blue. The green distribution is compatible with intracellular organellar staining, but no peroxisomal or mitochondrial marker was included to establish colocalization. After enzyme-mediated antigen retrieval and serum blocking, cells were incubated with both primary antibodies overnight at 4oC, followed by Fluoro488 and Cy3 secondary antibodies at 1:500.

ECH1 Antibody by IHC in human lung carcinoma. Immunohistochemistry of paraffin-embedded human lung carcinoma using Enoyl-CoA hydratase 1 Antibody reveals strong brown staining in gland-forming tumor epithelium. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB chromogen. Hematoxylin counterstains the nuclei blue.

ECH1 Antibody by WB in human and rodent samples. Western blot analysis using Enoyl-CoA hydratase 1 Antibody detects a prominent immunoreactive band near 30 kDa in human HepG2 (lane 1) and HeLa (lane 2) whole-cell lysates. The calculated molecular weight is approximately 36 kDa. Reduced samples containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody at 1:5000 and a chemiluminescent substrate. The band positions are described relative to the molecular weight standards in the blot.

ECH1 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of paraffin-embedded human thyroid carcinoma using Enoyl-CoA hydratase 1 Antibody reveals brown cytoplasmic staining in epithelial cells surrounding follicular spaces. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB chromogen. Hematoxylin counterstains the nuclei blue.

ECH1 Antibody by IHC in mouse heart. Immunohistochemistry of paraffin-embedded mouse heart using Enoyl-CoA hydratase 1 Antibody reveals brown staining in myocardial fibers. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB chromogen. Hematoxylin counterstains the nuclei blue.

ECH1 Antibody by IHC in rat heart. Immunohistochemistry of paraffin-embedded rat heart using Enoyl-CoA hydratase 1 Antibody reveals brown staining along cardiac muscle fibers. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB chromogen. Hematoxylin counterstains the nuclei blue.