- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ECE1 Antibody / Endothelin converting enzyme 1 (R32724)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R32724 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P42892 |
| Localization | Cytoplasm, cell membrane |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This ECE1 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
ECE1 antibody detects Endothelin Converting Enzyme 1, a membrane associated metalloprotease responsible for generating biologically active endothelin peptides that regulate vascular tone, cardiac function, and smooth muscle contraction. The UniProt recommended name is Endothelin-converting enzyme 1 (ECE1). As a key enzyme in the endothelin pathway, ECE1 converts inactive big endothelins into potent vasoactive endothelins, making it central to cardiovascular homeostasis, blood pressure regulation, and endothelial signaling.
ECE1 is a type I membrane protein composed of an extracellular zinc dependent catalytic domain, a single transmembrane region, and a cytoplasmic tail that regulates intracellular trafficking. The enzyme localizes primarily to the plasma membrane and Golgi associated compartments, where it processes precursor peptides during secretion or cell surface signaling events. Through these activities, ECE1 influences local concentrations of active endothelins that control vascular constriction, smooth muscle proliferation, and cardiovascular remodeling.
The ECE1 gene is located on chromosome 1p36.12 and produces multiple isoforms through alternative splicing. These isoforms differ in trafficking behavior and subcellular distribution, allowing fine tuned regulation of endothelin production in different tissues. ECE1 expression is especially prominent in endothelial cells, vascular smooth muscle, heart, lung, kidney, and brain. Its widespread distribution reflects the broad physiologic influence of endothelin signaling across vascular, renal, pulmonary, and neural systems.
Functionally, ECE1 plays a critical role in the endothelin axis, which regulates vascular resistance, cardiac output, and fluid balance. Active endothelin peptides produced by ECE1 bind endothelin receptors on vascular smooth muscle to trigger contraction and increase vascular tone. ECE1 also contributes to endothelial function by modulating autocrine and paracrine signaling pathways that influence nitric oxide balance, permeability, and vascular remodeling. Abnormal ECE1 activity can disrupt these finely tuned processes, contributing to cardiovascular dysfunction or dysregulated blood pressure responses.
Beyond vascular regulation, ECE1 influences development and tissue homeostasis. During embryogenesis, it contributes to formation of the cardiovascular system, craniofacial structures, and neural crest derived tissues. In adult tissues, ECE1 supports smooth muscle biology, extracellular matrix remodeling, and peptide processing pathways that govern local signaling networks. Through interactions with inflammatory and metabolic cues, ECE1 also participates in broader physiological processes, including renal sodium handling, pulmonary vascular control, and neuronal communication.
Pathologically, altered ECE1 activity has been associated with hypertension, heart failure, atherosclerosis, endothelial dysfunction, and pulmonary arterial hypertension. Excessive endothelin production can contribute to vascular stiffness, inflammatory signaling, and maladaptive fibrotic remodeling. Conversely, insufficient ECE1 activity can impair normal vascular reactivity or lead to developmental abnormalities. Genetic variants in ECE1 have been linked to congenital heart defects and craniofacial disorders. In oncology, ECE1 dysregulation has been observed in certain tumors, where aberrant endothelin signaling can influence proliferation, invasion, and angiogenesis.
ECE1 is also investigated for its roles in neurobiology. Active endothelins participate in neurotransmission, glial signaling, and neurovascular coupling. Because ECE1 affects local peptide availability, it is relevant to studies of neural regulation, pain pathways, and neuroinflammatory mechanisms.
ECE1 antibody supports research into endothelin pathway activity, vascular physiology, and protease mediated peptide processing. It is validated for use in relevant research applications to detect Endothelin Converting Enzyme 1 expression in cells and tissues. NSJ Bioreagents provides ECE1 antibody reagents suitable for cardiovascular research, developmental biology, renal physiology, and studies of vascular signaling.
Optimal dilution of the ECE1 antibody should be determined by the researcher.
Amino acids M18-T233 from the human protein were used as the immunogen for the ECE1 antibody.
After reconstitution, the ECE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
