EBNA1BP2 Antibody / Probable rRNA-processing protein EBP2 Antibody (FY13626)

EBNA1BP2 Antibody by IF in HeLa cells. Immunofluorescence analysis using Probable rRNA-processing protein EBP2 Antibody reveals green fluorescence concentrated in the nuclei, including bright nucleolar regions in HeLa cells. Red alpha-tubulin counterstaining highlights the cytoskeleton. Cells were treated with enzyme-based antigen retrieval and incubated with 5 ug/mL primary antibody overnight at 4oC before fluorescent secondary antibody detection. The image depicts the observed staining pattern without establishing organelle-specific colocalization.

EBNA1BP2 Antibody by IHC in human liver carcinoma. Immunohistochemistry of paraffin-embedded human liver carcinoma using Probable rRNA-processing protein EBP2 Antibody shows widespread brown staining within tumor cells with hematoxylin-counterstained nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0. Sections were blocked with goat serum and incubated with 2 ug/mL primary antibody overnight at 4oC, followed by HRP-conjugated secondary antibody and DAB visualization.

EBNA1BP2 Antibody by IHC in human lung carcinoma. Immunohistochemistry of paraffin-embedded human lung carcinoma using Probable rRNA-processing protein EBP2 Antibody shows brown staining in malignant epithelial cells with hematoxylin-counterstained nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0. Sections were blocked with goat serum and incubated with 2 ug/mL primary antibody overnight at 4oC, followed by HRP-conjugated secondary antibody and DAB visualization.

EBNA1BP2 Antibody by WB in human cell lines. Western blot analysis using Probable rRNA-processing protein EBP2 Antibody detects a prominent immunoreactive band consistent with the expected molecular weight of approximately 35 kDa. Lanes 1-4 contain A431, A549, HeLa and MCF-7 whole-cell lysates, respectively. Reduced samples containing 30 ug total protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk, incubated with 0.5 ug/mL primary antibody overnight at 4oC, and probed with an HRP-conjugated secondary antibody. Signal was visualized by chemiluminescence.

EBNA1BP2 Antibody by IP in A549. Immunoprecipitation of Probable rRNA-processing protein EBP2 Antibody from A549 whole-cell lysate was assessed by Western blot. Lane 1 contains 30 ug input lysate, lane 2 a rabbit IgG control immunoprecipitate, and lane 3 the antibody-specific immunoprecipitate prepared with 2 ug antibody and 500 ug lysate. An immunoreactive band consistent with the expected approximately 35 kDa protein is visible in input and the specific immunoprecipitate. The 50 kDa signal in immunoprecipitation lanes is consistent with immunoglobulin heavy chain.

EBNA1BP2 Antibody by FACS in A549 cells. Flow cytometry analysis using Probable rRNA-processing protein EBP2 Antibody demonstrates a rightward fluorescence shift in fixed and permeabilized A549 cells (blue) relative to rabbit IgG isotype control (green) and unstained blank (red). Cells were fixed in 4% paraformaldehyde, permeabilized, and incubated with 1 ug primary antibody per 1 x 10^6 cells for 30 minutes at 20oC before fluorescent secondary antibody staining. The experiment measures intracellular EBNA1BP2 rather than surface expression.