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Home >> Antibodies >> EBAG9 Antibody / Estrogen Receptor Binding Site Associated Antigen 9 / RCAS1

EBAG9 Antibody / Estrogen Receptor Binding Site Associated Antigen 9 / RCAS1 [clone CPTC-EBAG9-1] (V8199)

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  Catalog No Formulation Size Price (USD)  
Image V8199-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8199-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8199SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of permeabilized human MCF7 cells with EBAG9 antibody (clone CPTC-EBAG9-1, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of permeabilized human MCF7 cells with EBAG9 antibody (green) and Reddot nuclear stain (red).
IHC staining of FFPE human tonsil tissue with EBAG9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free EBAG9 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2c, kappa
Clone Name CPTC-EBAG9-1
Purity Protein G affinity chromatography
UniProt O00559
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This EBAG9 antibody is available for research use only.
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Description

EBAG9, also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.

Application Notes

Optimal dilution of the EBAG9 antibody should be determined by the researcher.

Immunogen

A recombinant human full-length EBAG9 protein was used as the immunogen for this EBAG9 antibody.

Storage

Store the EBAG9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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