E2F4 Antibody / Cell Cycle Regulation and Transcriptional Repressor Marker [clone E2F4/4224] (V4434)

E2F4 Antibody Lymph Node IHC. Immunohistochemistry analysis of FFPE human lymph node tissue stained with E2F4 antibody detecting E2F transcription factor 4, clone E2F4/4224. Strong nuclear staining is observed in lymphoid cells, consistent with the role of E2F4 as a transcriptional regulator within the RB pathway controlling cell cycle progression and gene repression. The staining highlights dense lymphocyte populations, while stromal areas show comparatively reduced signal. The inset shows a PBS-only negative control processed without primary antibody, confirming minimal non-specific background staining. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

E2F4 Antibody Breast Carcinoma IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue stained with E2F4 antibody detecting E2F transcription factor 4, clone E2F4/4224. Tumor epithelial cells show strong nuclear staining, consistent with localization of E2F4 as a transcriptional regulator within the RB pathway controlling cell cycle progression and gene repression. The staining highlights malignant glandular structures, while surrounding stromal regions show comparatively reduced signal. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

E2F4 Antibody HuProt Microarray. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using E2F4 antibody detecting E2F transcription factor 4, clone E2F4/4224. The antibody shows a strong and specific signal for E2F4 with a high Z-score and a significant separation from the next highest targets, resulting in an S-score greater than 2.5, consistent with high target specificity. Z-score represents the signal intensity in standard deviations above the array mean, while S-score reflects the difference between the top-ranked target and the next highest signal, indicating relative binding specificity of the monoclonal antibody.