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Home >> Antibodies >> E-Cadherin Antibody / CDH1

E-Cadherin Antibody / CDH1 [clone CDH1/3256] (V8269)

  Catalog No Formulation Size Price (USD)  
Image V8269-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8269-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8269SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Flow cytometry testing of human MCF7 cells with E-Cadherin antibody (clone CDH1/3256); Red=isotype control, Blue= E-Cadherin antibody.
IHC staining of FFPE human breast carcinoma with E-Cadherin antibody (clone CDH1/3256). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using E-Cadherin antibody (clone CDH1/3256). These results demonstrate the foremost specificity of the CDH1/3256 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free E-Cadherin antibody (clone CDH1/3256) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CDH1/3256
Purity Protein G affinity chromatography
UniProt P12830
Localization Cell surface
Applications Flow cytometry : 1-2ug/million cells in 0.1ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This E-Cadherin antibody is available for research use only.
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Description

Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Application Notes

Optimal dilution of the E-Cadherin antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 567-691) was used as the immunogen for this E-Cadherin antibody.

Storage

Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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