E-cadherin Antibody for WB / Developmental Epithelial Marker Western Blot Antibody [clone 6B10-F4-G10] (F54046)

E-cadherin Antibody for WB. Western blot analysis of Cadherin 1 / CDH1 expression in human cell lysates: 1) T47D, 2) HCT116, 3) MDA-MB-468, and 4) Caco-2 using clone 6B10-F4-G10 antibody at 1:2000, showing bands near the predicted molecular weight of E-cadherin. E-cadherin is synthesized as a precursor and undergoes glycosylation, resulting in an apparent molecular weight typically observed between approximately 80-120 kDa, with the precursor form near 135 kDa. The detected bands are consistent with mature glycosylated E-cadherin across epithelial-derived cell lines, with variation in band intensity reflecting differences in expression levels.

E-cadherin Antibody for WB in mouse embryo and human HCT116 cells. Western blot analysis of Cadherin 1 / CDH1 expression in 1) mouse embryo and 2) human HCT116 cell lysates using clone 6B10-F4-G10 antibody, showing bands near the predicted molecular weight of E-cadherin. E-cadherin is synthesized as a precursor and undergoes glycosylation, resulting in an apparent molecular weight typically observed between approximately 80-120 kDa, with the precursor form near 135 kDa. The detected bands are consistent with mature glycosylated E-cadherin, with stronger signal in HCT116 cells reflecting higher epithelial expression.