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Home >> Antibodies >> E-Cadherin Antibody

E-Cadherin Antibody [clone 6B10-F4-G10] (F54046)

  Catalog No Formulation Size Price (USD)  
Image F54046-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot testing of human 1) T47D, 2) HCT116, 3) MDA-MB-468 and 4) Caco-2 cell lysates using E-Cadherin antibody at 1:2000. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Western blot testing of 1) mouse embryo and 2) human HCT116 cell lysates using E-Cadherin antibody. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 6B10-F4-G10
Purity Protein G affinity
UniProt P12830
Applications Western blot : 1:1000
Limitations This E-Cadherin antibody is available for research use only.
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Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Storage

Store the E-Cadherin antibody at -20oC.

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