- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cadherin 1 Antibody for IF / E-cadherin Immunofluorescence Antibody - Epithelial Cell Boundary Marker [clone 4A2] (V3232)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V3232-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V3232-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V3232SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | 4A2 |
| Purity | Protein G affinity chromatography |
| UniProt | P12830 |
| Localization | Cytoplasmic, membranous |
| Applications | Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-4ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Cadherin 1 Antibody for IF / E-cadherin Immunofluorescence Antibody - Epithelial Cell Boundary Marker is available for research use only. |
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Cadherin 1 (CDH1), commonly known as E-cadherin, is a transmembrane adhesion protein that defines epithelial cell boundaries through calcium-dependent homophilic interactions. CDH1 localizes to the lateral plasma membrane, where it forms adherens junctions that establish clear separation between neighboring cells and maintain epithelial organization. Cadherin 1 Antibody for IF enables precise visualization of these cell boundaries in cultured cells, making it an essential tool for studying epithelial morphology and cellular arrangement. CDH1 is widely referred to as E-cadherin antibody and serves as a key marker of epithelial identity and structural organization.
Cadherin 1 Antibody for IF / E-cadherin Immunofluorescence Antibody - Epithelial Cell Boundary Marker (clone 4A2) is specifically optimized for imaging applications that emphasize delineation of individual cell borders. The mouse monoclonal clone 4A2 antibody produces strong and well-defined membrane staining in epithelial cell models such as MCF7, clearly outlining each cell and enabling accurate visualization of cell perimeter and morphology. This clone 4A2 antibody supports consistent fluorescence imaging and is particularly useful for studies requiring high-contrast boundary definition.
In immunofluorescence assays, Cadherin 1 antibody generates a distinct membrane staining pattern that traces the outline of each cell, allowing detailed assessment of cell shape, size, and arrangement within epithelial monolayers. This boundary-focused visualization is critical for analyzing cell packing density, epithelial sheet organization, and morphological changes under different experimental conditions. Uniform and continuous staining indicates intact epithelial structure, while irregular or fragmented boundaries may reflect altered adhesion or cellular transformation.
Clear visualization of cell boundaries is essential for understanding epithelial dynamics, including proliferation, migration, and differentiation. E-cadherin plays a central role in maintaining these boundaries through its interactions with intracellular proteins such as beta-catenin, which link adhesion complexes to the actin cytoskeleton. Cadherin 1 immunofluorescence antibody enables visualization of these boundary-defining structures and supports studies of epithelial organization at the single-cell level.
This Cadherin 1 antibody is particularly effective for fluorescence-based imaging workflows focused on cell boundary definition and epithelial morphology. The sharp and continuous membrane staining produced by clone 4A2 allows precise interpretation of cellular organization and supports detailed analysis of epithelial structure and arrangement.
This antibody is part of the CDH1 antibody collection, where multiple E-cadherin antibody formats and applications are available for studying epithelial adhesion and cancer progression.
Optimal dilution of the Cadherin 1 Antibody for IF / E-cadherin Immunofluorescence Antibody - Epithelial Cell Boundary Marker should be determined by the researcher.
A recombinant human protein was used as the immunogen for the E-Cadherin/Cadherin 1 antibody.
Store the E-Cadherin/Cadherin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
E-cadherin antibody, CDH1 antibody, Cadherin 1 IF antibody, cell boundary marker antibody, epithelial border antibody
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