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Home >> Antibodies >> E-Cadherin Antibody

E-Cadherin Antibody [clone 4A2] (V3232)

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  Catalog No Formulation Size Price (USD)  
Image V3232-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3232-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3232SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (green, clone 4A2) and Reddot nuclear stain (red).
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Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (green, clone 4A2) and Reddot nuclear stain (red).
IHC testing of FFPE human skin with E-Cadherin antibody (clone 4A2). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Western blot testing of human MCF-7 cell lysate with E-Cadherin antibody (clone 4A2). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Flow cytometry testing of human MCF7 cells with E-Cadherin antibody (clone 4A2); Red=isotype control, Blue= E-Cadherin antibody.
SDS-PAGE analysis of purified, BSA-free E-Cadherin antibody (clone 4A2) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 4A2
Purity Protein G affinity chromatography
UniProt P12830
Localization Cytoplasmic, membranous
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-4ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This E-Cadherin antibody is available for research use only.
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Description

Recognizes a protein of 80-120kDa, identified as E-cadherin/CDH1. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Application Notes

Optimal dilution of the E-Cadherin antibody should be determined by the researcher.

Immunogen

A recombinant human protein was used as the immunogen for the E-Cadherin antibody.

Storage

Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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