DYSF Antibody / Dysferlin Membrane Repair Protein Antibody (RQ6473)

DYSF Antibody Skeletal Muscle IHC. Immunohistochemistry staining of FFPE human skeletal muscle tissue using DYSF Antibody demonstrates widespread cytoplasmic and sarcolemmal-associated HRP-DAB brown staining throughout skeletal muscle fibers. The staining pattern is consistent with expression of Dysferlin / DYSF, a ferlin family membrane repair protein that supports calcium-dependent sarcolemmal maintenance and recovery following muscle fiber injury. Signal is distributed along elongated myofibers with membranous accentuation, reflecting the established role of Dysferlin in membrane remodeling and preservation of muscle integrity in mechanically active tissues. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

DYSF Antibody Skeletal Muscle Tissue IHC. Immunohistochemistry staining of FFPE mouse skeletal muscle tissue using DYSF Antibody demonstrates strong cytoplasmic and sarcolemmal-associated HRP-DAB brown staining throughout skeletal muscle fibers. The staining pattern is consistent with expression of Dysferlin / DYSF, a membrane repair protein that supports calcium-dependent sarcolemmal maintenance and recovery following contraction-induced membrane injury. Prominent staining within elongated myofibers highlights the established role of Dysferlin in membrane remodeling, muscle fiber stability, and preservation of skeletal muscle integrity. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

DYSF Antibody Multi-Species Heart WB. Western blot analysis of Lane 1: human PC-3 cell lysate, Lane 2: rat heart lysate, and Lane 3: mouse heart lysate using DYSF Antibody / Dysferlin Membrane Repair Protein Antibody. A band is detected at approximately 250-260 kDa in all three samples, corresponding to Dysferlin / DYSF. The observed molecular weight is modestly higher than the predicted molecular weight of approximately 237 kDa, which can occur with large membrane-associated proteins. Dysferlin is a ferlin family membrane repair protein that mediates calcium-dependent sarcolemmal restoration and maintenance of muscle cell integrity. Detection in rat and mouse heart lysates is consistent with the established expression of Dysferlin in contractile tissues that undergo continual mechanical stress and membrane remodeling.

DYSF Antibody HL60 FACS. Flow cytometric analysis of human HL60 cells using DYSF Antibody demonstrates a rightward shift of the blue population relative to the isotype control (green), consistent with detection of Dysferlin / DYSF expression. Dysferlin is a ferlin family membrane repair protein that participates in calcium-dependent membrane remodeling and cellular recovery following membrane injury. Cells were stained with 1 ug antibody per million cells following blocking with goat sera. Red = cells alone, Green = isotype control, Blue = DYSF antibody.

Dysferlin Antibody IF/ICC. Immunofluorescence analysis of SiHa cells using Dysferlin Antibody demonstrates distinct cytoplasmic and membranous green fluorescence consistent with the established localization of Dysferlin (DYSF), a membrane-associated protein involved in sarcolemmal repair, vesicle trafficking, and membrane fusion processes. Signal is distributed throughout the cytoplasm with enrichment along cellular membranes, while nuclei are counterstained with DAPI (blue). The observed staining pattern supports studies of Dysferlin (DYSF) function, skeletal muscle membrane repair, sarcolemmal integrity, vesicle trafficking, muscular dystrophy research, and muscle fiber regeneration pathways. Enzyme antigen retrieval was performed for 15 minutes prior to staining. Target protein is visualized in green and nuclei are shown in blue.