DYRK3 Antibody / Dual specificity tyrosine-phosphorylation-regulated kinase 3 Antibody (FY13570)

DYRK3 Antibody by IF in U2OS cells. Immunofluorescence using Dual specificity tyrosine-phosphorylation-regulated kinase 3 Antibody shows green DYRK3-associated signal within nuclei and surrounding cytoplasm of U2OS cells, together with red alpha-tubulin staining of the cytoskeleton. Published research documents both nuclear and cytoplasmic localization of DYRK3. Cells were treated with enzyme antigen retrieval reagent for 15 minutes and blocked with 10% goat serum. Rabbit primary antibody against DYRK3 and mouse anti-alpha-tubulin were each incubated overnight at 4oC. Fluoro488- and Cy3-conjugated secondary antibodies were used at 1:500.

DYRK3 Antibody by WB in human K562. Western blot analysis using Dual specificity tyrosine-phosphorylation-regulated kinase 3 Antibody detects an immunoreactive band near 66 kDa in human K562 (lane 1); human SiHa (lane 2); human Jurkat (lane 3); human U251 (lane 4) whole-cell lysates. The calculated molecular weight is approximately 66 kDa. Reduced lysates containing 30 ug protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC and developed with an HRP-conjugated secondary antibody and chemiluminescence.