Deoxyuridine Triphosphatase Antibody / DUT (R35363)

Deoxyuridine Triphosphatase Antibody HEK293 and HeLa WB. Western blot analysis of HEK293 and HeLa cell lysates using Deoxyuridine Triphosphatase Antibody demonstrates detection of a band at approximately 18 kDa, consistent with the mature nuclear isoform of DUT. Deoxyuridine Triphosphatase is a nucleotide metabolism enzyme that regulates intracellular dUTP pools and prevents inappropriate uracil incorporation into DNA, thereby supporting DNA replication fidelity and genome maintenance. DUT is known to exist as multiple isoforms, including a longer precursor form and a shorter processed nuclear form. The observed band is consistent with the mature nuclear isoform commonly detected in proliferating human cells. Western blot was performed using 0.1 ug/ml primary antibody. Predicted molecular weights: ~27 kDa (isoform 1) and ~18 kDa (isoform 2).