DUT Antibody / Deoxyuridine triphosphatase (FY13460)

IHC analysis of DUT Antibody / Deoxyuridine triphosphatase. DUT expression was examined in a paraffin-embedded section of human tonsil tissue. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with goat serum and incubated with a rabbit anti-DUT antibody. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen. Prominent nuclear staining is observed in lymphoid cells within germinal center regions, consistent with the role of DUT in DNA replication and nucleotide metabolism in proliferating cells.

IHC analysis of DUT Antibody / Deoxyuridine triphosphatase. DUT expression was examined in a paraffin-embedded section of human tonsil tissue. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with goat serum and incubated with a rabbit anti-DUT antibody. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen. Nuclear-predominant staining is observed in lymphoid cells, with variable intensity reflecting differences in cellular proliferation states across tissue regions.

IHC analysis of DUT Antibody / Deoxyuridine triphosphatase. DUT expression was examined in a paraffin-embedded section of human tonsil tissue. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with goat serum and incubated with a rabbit anti-DUT antibody. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen. Broad nuclear staining is observed across lymphoid compartments, providing tissue-level context for DUT expression in immune cells.

IF analysis of DUT Antibody / Deoxyuridine triphosphatase. DUT localization was examined in immunocytochemical sections of human HeLa cells. Cells were blocked with goat serum and incubated with a rabbit anti-DUT antibody together with an alpha tubulin antibody. DUT signal is shown in green, alpha tubulin in red. DUT staining is predominantly nuclear, consistent with its role in regulating nucleotide pools during DNA replication, while alpha tubulin outlines the cytoplasmic microtubule network.

Western blot analysis of DUT Antibody / Deoxyuridine triphosphatase. Proteins were separated by 12% SDS-PAGE and transferred to a nitrocellulose membrane for immunodetection. Lane 1: human HEL whole cell lysates; Lane 2: human Jurkat whole cell lysates; Lane 3: human SH-SY5Y whole cell lysates; Lane 4: human HeLa whole cell lysates. A predominant band is detected at approximately 18 kDa across all samples. Although DUT is reported to have an apparent molecular weight of approximately 27 kDa for the full-length precursor, the lower band observed here is consistent with the mature, processed nuclear dUTPase isoform. DUT is known to produce multiple isoforms through alternative targeting and proteolytic processing, including a shorter nuclear form and a longer mitochondrial precursor, which explains the observed size differences across experimental systems.