DUT Antibody / Genome Integrity Enzyme Antibody (FY13460)

DUT Antibody Human Tonsil IHC. Immunohistochemistry staining of FFPE human tonsil using DUT antibody demonstrates prominent nuclear staining within proliferative lymphoid cell populations of the germinal center region, consistent with the expected localization of Deoxyuridine Triphosphatase (DUT). DUT is a genome integrity enzyme that regulates intracellular nucleotide pools and prevents inappropriate uracil incorporation into DNA during replication. The observed nuclear staining pattern is consistent with elevated nucleotide metabolism and DNA replication activity in actively proliferating lymphocytes and supports the utility of this antibody for studies of genome maintenance, DNA replication fidelity, and cellular proliferation pathways. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

DUT Antibody Human Tonsil Germinal Center IHC. Immunohistochemistry staining of FFPE human tonsil using DUT antibody demonstrates prominent nuclear staining within germinal center lymphocytes, consistent with the expected localization of Deoxyuridine Triphosphatase (DUT). DUT is a genome integrity enzyme that maintains nucleotide pool balance and prevents inappropriate uracil incorporation into DNA during replication. The strong nuclear staining observed within actively proliferating lymphoid cells is consistent with increased demand for nucleotide metabolism and DNA replication fidelity in germinal center reactions. Variable staining intensity among cellular populations reflects differences in proliferative activity and supports the utility of this antibody for studies of genome maintenance, cellular proliferation, and DNA integrity pathways. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

DUT Antibody Human Tonsil IHC. Immunohistochemistry staining of FFPE human tonsil using DUT antibody demonstrates widespread nuclear staining throughout lymphoid compartments, with particularly prominent immunoreactivity within germinal center-associated cell populations. DUT (Deoxyuridine Triphosphatase) is a genome integrity enzyme that regulates intracellular nucleotide pools and protects DNA from inappropriate uracil incorporation during replication. The broad nuclear staining pattern observed across proliferative immune cell populations is consistent with the established role of DUT in supporting DNA replication fidelity, genome maintenance, and cellular proliferation. These findings support the utility of DUT antibody for studies of nucleotide metabolism, DNA integrity pathways, and lymphoid tissue biology. HIER: heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

DUT Antibody HeLa Cell IF. Immunofluorescent staining of human HeLa cells using DUT antibody demonstrates predominant nuclear localization of Deoxyuridine Triphosphatase (DUT), shown in green, consistent with its role in regulating nucleotide pools and maintaining DNA replication fidelity. DUT is a genome integrity enzyme that prevents inappropriate uracil incorporation into DNA and supports genomic stability during cellular proliferation. Co-staining with alpha tubulin (red) highlights the cytoplasmic microtubule network and provides contrast to the nuclear distribution of DUT. The observed staining pattern is consistent with the established function of DUT in nucleotide metabolism, genome maintenance, and DNA integrity pathways. Alpha tubulin was used as a cytoplasmic marker.

DUT Antibody Human Cell Line WB. Western blot analysis of DUT using anti-DUT antibody demonstrates a predominant immunoreactive band at approximately 18 kDa in human HEL, Jurkat, SH-SY5Y, and HeLa cell lysates. DUT (Deoxyuridine Triphosphatase) is a genome integrity enzyme that regulates intracellular nucleotide pools and prevents inappropriate uracil incorporation into DNA during replication. The observed lower molecular weight species is consistent with the mature nuclear dUTPase isoform, which results from intracellular processing of the DUT precursor protein. DUT is known to exist as multiple isoforms, including nuclear and mitochondrial forms, reflecting its diverse roles in nucleotide metabolism, DNA replication fidelity, and genome maintenance. Predicted molecular weight: ~27 kDa precursor; mature nuclear isoform commonly observed at approximately 18 kDa.