DUSP6 Antibody / MAPK Signaling Regulator Antibody (RQ6707)

DUSP6 Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue using DUSP6 antibody demonstrates diffuse cytoplasmic HRP-DAB brown staining throughout neuronal-rich regions of the brain. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. By dephosphorylating activated ERK proteins, DUSP6 helps control signaling intensity and duration, thereby regulating cellular responses associated with differentiation, survival, and tissue homeostasis. The observed neural expression pattern is consistent with the importance of tightly regulated MAPK signaling in nervous system function and cellular communication. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

DUSP6 Antibody Human Lymphadenoma IHC. Immunohistochemical staining of FFPE human lymphadenoma tissue using DUSP6 antibody demonstrates widespread cytoplasmic HRP-DAB brown staining throughout neoplastic lymphoid cell populations. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. By controlling the phosphorylation state of ERK proteins, DUSP6 helps modulate signaling networks involved in cellular proliferation, differentiation, survival, and responses to growth factor stimulation. The observed staining supports the role of DUSP6 in regulating intracellular signaling pathways that influence cellular homeostasis and tissue biology. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

DUSP6 Antibody Human Colonic Adenocarcinoma IHC. Immunohistochemical staining of FFPE human colonic adenocarcinoma tissue using DUSP6 antibody demonstrates moderate to strong cytoplasmic HRP-DAB brown staining within neoplastic gland-forming epithelial cells. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. DUSP6 modulates cellular responses to growth factor stimulation by controlling ERK phosphorylation and signaling duration, thereby influencing pathways associated with cellular proliferation, differentiation, and survival. The observed staining within tumor epithelial structures supports the role of DUSP6 in regulating intracellular signaling networks that contribute to tissue homeostasis and cellular growth control. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

DUSP6 Antibody Human Rectal Cancer IHC. Immunohistochemical staining of FFPE human rectal cancer tissue using DUSP6 antibody demonstrates moderate cytoplasmic HRP-DAB brown staining within neoplastic glandular epithelial cells. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. By dephosphorylating activated ERK proteins, DUSP6 helps control signaling intensity and duration, thereby influencing cellular proliferation, differentiation, survival, and responses to growth factor stimulation. The observed expression within malignant glandular structures supports the role of DUSP6 in regulating intracellular signaling networks that contribute to tissue homeostasis and cellular growth control. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

DUSP6 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using DUSP6 antibody demonstrates moderate membranous and cytoplasmic HRP-DAB brown staining within neoplastic hepatic cell populations. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. DUSP6 modulates cellular responses to growth factor stimulation by controlling ERK phosphorylation and signaling duration, thereby influencing pathways associated with cellular proliferation, differentiation, survival, and tissue homeostasis. The observed expression within tumor cells supports the role of DUSP6 in regulating intracellular signaling networks that govern cellular growth and signal transduction. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

DUSP6 Antibody Human Liver Cancer Tissue IHC. Immunohistochemical staining of FFPE human liver cancer tissue using DUSP6 antibody demonstrates diffuse cytoplasmic HRP-DAB brown staining throughout neoplastic hepatic cell populations. The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. By dephosphorylating activated ERK proteins, DUSP6 helps modulate signaling intensity and duration, thereby influencing cellular proliferation, differentiation, survival, and responses to growth factor stimulation. The widespread staining observed within tumor cells supports the role of DUSP6 in maintaining signaling homeostasis within pathways that regulate cellular growth and tissue biology. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

DUSP6 Antibody PC-3 Cell IF. Immunofluorescent staining of FFPE human PC-3 cells using DUSP6 antibody demonstrates prominent cytoplasmic green fluorescence with relative exclusion from DAPI-positive nuclei (blue). The staining pattern is consistent with expression of DUSP6, a dual-specificity phosphatase that functions as a key negative regulator of ERK/MAPK signaling pathways. DUSP6 modulates signal transduction by dephosphorylating activated ERK proteins, thereby helping control signaling intensity, duration, and downstream cellular responses. The predominantly cytoplasmic localization observed in PC-3 cells is consistent with the established role of DUSP6 in regulating MAPK pathway activity within the cytoplasmic compartment. HIER: steam section in pH 6 citrate buffer for 20 minutes.

DUSP6 Antibody Multi-Species Western Blot. Western blot analysis of human U-87 MG (lane 1), RT4 (lane 2), PC-3 (lane 3), HL-60 (lane 4), HepG2 (lane 5), rat brain (lane 6), and mouse brain (lane 7) lysates using DUSP6 antibody demonstrates prominent bands migrating at approximately 50-60 kDa across all tested samples. DUSP6 (Dual Specificity Phosphatase 6) is a key negative regulator of ERK/MAPK signaling pathways, functioning to dephosphorylate activated ERK proteins and thereby modulate signal transduction, cellular differentiation, proliferation, and growth factor responses. Detection of DUSP6 in multiple human cell lines and rodent brain tissues supports its broad role in maintaining MAPK signaling homeostasis across diverse tissue types and biologic systems. Predicted molecular weights are approximately 42 kDa and 26 kDa for DUSP6 isoforms 1 and 2, respectively.

DUSP6 Antibody MAPK Signaling Regulator FACS. Flow cytometric analysis of human HepG2 cells using DUSP6 antibody demonstrates a pronounced rightward shift of the positive cell population (blue) relative to both the isotype control (green) and unstained cells (red), consistent with expression of DUSP6. DUSP6 (Dual Specificity Phosphatase 6) functions as a key negative regulator of ERK/MAPK signaling pathways by dephosphorylating activated ERK proteins and modulating downstream signal transduction. Through this activity, DUSP6 helps regulate cellular responses to growth factor stimulation, differentiation cues, and proliferative signals. The observed fluorescence shift supports the utility of this antibody for flow cytometric detection of DUSP6 expression. Red=cells alone; Green=isotype control; Blue=DUSP6 antibody.