DUSP6 Antibody (F51142)

DUSP6 Antibody Brain Tissue Western Blot. Western blot analysis of human brain (lane 1), mouse brain (lane 2), and rat brain (lane 3) tissue lysates using DUSP6 antibody demonstrates distinct bands at approximately 42 kDa, consistent with the expected molecular weight of DUSP6 isoform 1. DUSP6 (Dual Specificity Phosphatase 6) is a key negative regulator of ERK/MAPK signaling pathways, functioning to dephosphorylate activated ERK proteins and modulate downstream signal transduction. By regulating signaling intensity and duration, DUSP6 influences pathways involved in cellular differentiation, survival, growth factor responses, and tissue homeostasis. Detection across human, mouse, and rat brain tissues supports the conserved role of DUSP6 in maintaining MAPK signaling balance within the nervous system. Predicted molecular weights for DUSP6 isoforms are approximately 42 kDa and 26 kDa.

DUSP6 Antibody Human and Brain Tissue Western Blot. Western blot analysis of human HepG2 cells (lane 1), human brain tissue (lane 2), and rat brain tissue (lane 3) using DUSP6 antibody demonstrates distinct bands at approximately 42-45 kDa, consistent with the expected molecular weight of DUSP6 isoform 1. DUSP6 (Dual Specificity Phosphatase 6) is a key negative regulator of ERK/MAPK signaling pathways, functioning to dephosphorylate activated ERK proteins and thereby modulate signal transduction, growth factor responses, cellular differentiation, and tissue homeostasis. Detection of DUSP6 in both hepatic and neural tissues supports its broad role in maintaining MAPK signaling balance across diverse biologic systems. Predicted molecular weights for DUSP6 isoforms are approximately 42 kDa and 26 kDa.

DUSP6 Antibody HeLa Cell Confocal IF. Confocal immunofluorescent analysis of HeLa cells using DUSP6 antibody demonstrates prominent cytoplasmic green fluorescence surrounding DAPI-stained nuclei (blue), consistent with expression of DUSP6. DUSP6 (Dual Specificity Phosphatase 6) is a key negative regulator of ERK/MAPK signaling pathways that functions by dephosphorylating activated ERK proteins and controlling downstream signal transduction. The predominantly cytoplasmic localization observed in HeLa cells is consistent with the established role of DUSP6 in regulating MAPK pathway activity and maintaining signaling homeostasis. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used for signal detection, and nuclei were counterstained with DAPI.

DUSP6 Antibody Human Kidney IHC. Immunohistochemical staining of FFPE human kidney tissue using DUSP6 antibody demonstrates strong cytoplasmic HRP-DAB brown staining within renal tubular epithelial cells, with comparatively weaker staining in surrounding stromal and glomerular structures. The staining pattern is consistent with expression of DUSP6 (Dual Specificity Phosphatase 6), a key negative regulator of ERK/MAPK signaling pathways. By dephosphorylating activated ERK proteins, DUSP6 helps modulate intracellular signaling responses involved in cellular differentiation, survival, growth factor signaling, and tissue homeostasis. The prominent tubular epithelial staining supports the role of DUSP6 in maintaining regulated MAPK pathway activity within metabolically active epithelial tissues. HIER: steam section in pH 9 EDTA for 20 minutes and allow to cool prior to staining.

DUSP6 Antibody Human Colon Carcinoma IHC. Immunohistochemical staining of FFPE human colon carcinoma tissue using DUSP6 antibody demonstrates strong and widespread cytoplasmic HRP-DAB brown staining throughout neoplastic gland-forming epithelial cells. The staining pattern is consistent with expression of DUSP6 (Dual Specificity Phosphatase 6), a key negative regulator of ERK/MAPK signaling pathways. DUSP6 modulates intracellular signal transduction by dephosphorylating activated ERK proteins, thereby helping regulate cellular responses to growth factor stimulation, differentiation signals, and proliferative cues. The robust staining observed within malignant epithelial structures supports the role of DUSP6 in maintaining signaling homeostasis within pathways that influence cellular growth and tissue organization. HIER: steam sections in pH 9 EDTA for 20 minutes and allow to cool prior to staining.