DUSP2 Antibody / Dual specificity protein phosphatase 2 (FY13443)

Immunofluorescence localization of DUSP2 in HeLa cells. DUSP2 expression was analyzed in cultured HeLa cells by immunofluorescence. Cells were fixed, subjected to enzymatic antigen retrieval, and incubated with an anti-DUSP2 antibody together with an alpha-tubulin antibody as a cytoskeletal marker. Fluorescent secondary antibodies were used for detection. DUSP2 staining is visualized in green, alpha-tubulin in red.

Immunohistochemistry of DUSP2 in human tonsil tissue. DUSP2 expression was examined in a paraffin-embedded section of human tonsil. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0). After blocking with normal serum, the section was incubated with an anti-DUSP2 antibody, followed by an HRP-conjugated secondary antibody and DAB chromogen. Brown staining indicates DUSP2 immunoreactivity, with hematoxylin counterstaining highlighting tissue architecture.

Western blot analysis of DUSP2 in rodent lymphoid tissues. DUSP2 expression was examined by western blot in rat spleen, rat thymus, mouse spleen, and mouse thymus tissue lysates resolved by SDS-PAGE and transferred to a nitrocellulose membrane. A prominent band corresponding to full-length DUSP2 was detected at approximately 34 kDa, consistent with the predicted molecular weight. One or two weaker lower-molecular-weight bands were also observed, which are consistent with previously reported truncated or proteolytically processed DUSP2 species in lymphoid tissues. Stronger signal in spleen and thymus reflects expected immune-enriched expression of DUSP2.

Flow cytometry analysis of fixed and permeabilized human RT4 cells with DUSP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DUSP2 antibody.