DUSP18 Antibody / Dual specificity protein phosphatase 18 Antibody (FY13640)

DUSP18 Antibody by IF in U2OS cells. Immunofluorescence analysis using Dual specificity protein phosphatase 18 Antibody reveals green nuclear-enriched fluorescence in U2OS cells, with red alpha-tubulin staining outlining the cytoskeleton. Cells underwent enzyme antigen retrieval and were incubated with 5 ug/mL primary antibody overnight at 4oC, followed by fluorophore-conjugated secondary antibodies. The figure documents nuclear-enriched staining but does not establish a mechanism for the observed distribution.

DUSP18 Antibody by WB in Jurkat cells. Western blot analysis using Dual specificity protein phosphatase 18 Antibody detects a prominent band consistent with the expected molecular weight of approximately 21 kDa in human Jurkat whole-cell lysate (lane 1). Reduced lysate containing 30 ug total protein was resolved by 12% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk and incubated with 0.5 ug/mL primary antibody overnight at 4oC, followed by an HRP-conjugated secondary antibody and chemiluminescent detection.