DUSP12 Antibody / Dual specificity protein phosphatase 12 Antibody (FY13607)

DUSP12 Antibody by IF in HeLa cells. Immunofluorescence staining of HeLa cells using Dual specificity protein phosphatase 12 Antibody reveals strong green nuclear staining with a weaker surrounding cytoplasmic signal. Target immunoreactivity is shown in green, with alpha-tubulin labeling in red. The cells underwent enzyme-based antigen retrieval, blocking with 10% goat serum, and overnight primary antibody incubation at 5 ug/mL at 4oC. Fluorescent secondary antibodies were applied at 1:500 before microscopy.

DUSP12 Antibody by FACS in SH-SY5Y cells. Flow cytometry of fixed, permeabilized SH-SY5Y cells using Dual specificity protein phosphatase 12 Antibody shows a rightward fluorescence shift in the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and blank control (red). Cells were fixed in 4% paraformaldehyde, permeabilized, and incubated with 1 ug primary antibody per 1 x 10^6 cells. A Fluoro488-conjugated secondary antibody was used for detection. This assay measures intracellular immunoreactivity rather than surface expression.

DUSP12 Antibody by WB in human cell lines. Western blot analysis using Dual specificity protein phosphatase 12 Antibody detects a band near 36 kDa in SH-SY5Y (lane 1), U251 (lane 2), PC-3 (lane 3), and Caco-2 (lane 4) whole-cell lysates. The observed migration is compared with the calculated molecular weight of approximately 38 kDa. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and chemiluminescence.