DUSP1 Antibody / MAPK Signaling Regulator Antibody (R36116)

DUSP1 Antibody HeLa WB. Western blot analysis of DUSP1 expression was performed using anti-DUSP1 antibody in human HeLa cell lysate. DUSP1, also known as Dual Specificity Phosphatase 1 or MKP1 (MAP Kinase Phosphatase 1), is a stress-inducible phosphatase that negatively regulates MAPK signaling through dephosphorylation of ERK, JNK, and p38 kinases. A specific immunoreactive band is detected at approximately 39 kDa, corresponding to the predicted molecular weight of DUSP1. Expression in HeLa cells is consistent with the established role of DUSP1 as a feedback regulator of cellular stress responses, inflammatory signaling, and growth factor-mediated pathways. The blot was performed using DUSP1 antibody at 0.3 ug/ml. These results support the utility of DUSP1 Antibody for studies of MAPK regulation, signal transduction, cellular adaptation, and stress-responsive signaling networks.

DUSP1 Antibody Human Prostate IHC. IHC staining of FFPE human prostate tissue with DUSP1 antibody demonstrated strong cytoplasmic staining of glandular epithelial cells, consistent with the expression pattern of DUSP1 (Dual Specificity Phosphatase 1), also known as MKP1. DUSP1 is a stress-inducible phosphatase that negatively regulates MAPK signaling by dephosphorylating ERK, JNK, and p38 kinases, thereby controlling cellular proliferation, differentiation, and stress responses. The prominent epithelial staining observed is consistent with the role of DUSP1 in maintaining signaling homeostasis within secretory epithelial tissues. Immunostaining was performed using DUSP1 antibody at 4 ug/ml with an alkaline phosphatase detection system. Heat-induced epitope retrieval was achieved by steaming tissue sections in pH 6 citrate buffer prior to staining. These results support the utility of DUSP1 Antibody for studies of MAPK regulation, signal transduction, cellular stress responses, and prostate biology.