DTYMK Antibody / Thymidylate kinase Antibody (FY13652)

DTYMK Antibody by IF in HeLa cells. Immunofluorescence staining of fixed HeLa cells with DTYMK Antibody shows green signal concentrated in the cytoplasmic and perinuclear regions, surrounding blue nuclear counterstaining. The image shows several cells with uneven cytoplasmic staining intensity. The staining pattern is described from the image without assigning it to a specific organelle, since no colocalization marker is shown. The Thymidylate kinase Antibody signal is visualized in green.

DTYMK Antibody by IHC in human liver cancer tissue. Immunohistochemistry of paraffin-embedded human liver cancer tissue using DTYMK Antibody, with antigen retrieval in EDTA buffer (pH 8.0) and blocking in 10% goat serum. The section was incubated with rabbit primary antibody at 2 ug/ml overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB development. Strong brown staining is visible in tumor cell regions with blue counterstained nuclei. This experiment illustrates detection using the DTYMK antibody under the indicated staining conditions.

DTYMK Antibody by WB in 3 lysate samples. Western blot analysis using DTYMK Antibody was performed with 30 ug of protein per lane under reducing conditions on a 12% SDS-PAGE gel. Lane assignments: 1, human A549 whole-cell lysate; 2, human HeLa whole-cell lysate; 3, human PC-3 whole-cell lysate. Following electrophoresis, proteins were transferred to nitrocellulose and blocked with 5% non-fat milk. The membrane was incubated with rabbit primary antibody at 0.5 ug/ml overnight at 4oC and HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5000). ECL detection showed an immunoreactive band near 24 kDa, consistent with the approximately 23.8 kDa expected size of DTYMK.