DTNA Antibody / Dystrobrevin alpha Antibody (FY13575)

DTNA Antibody by IF in U2OS cells. Immunofluorescence using Dystrobrevin alpha Antibody shows green punctate cytoplasmic staining in U2OS cells, particularly in regions adjacent to the nuclei, which are counterstained blue with DAPI. The signal is largely extranuclear in this preparation. Cells underwent enzyme-mediated antigen retrieval for 15 minutes, were blocked with 10% goat serum, and incubated with primary antibody at 5 ug/mL overnight at 4oC. Fluoro488-conjugated goat anti-rabbit IgG was used at 1:500 for 30 minutes at 37oC.

DTNA Antibody by WB in human cancer cell lines and rodent brain. Western blot analysis using Dystrobrevin alpha Antibody detects a prominent immunoreactive band near 84 kDa in human MCF-7 (lane 1), human U251 (lane 2), rat brain (lane 3), and mouse brain (lane 4). The calculated molecular weight is approximately 83.9 kDa. Additional higher-molecular-weight bands are visible in the human and mouse samples, but their identities are not established in this experiment. Samples were analyzed under reducing conditions with 30 ug protein per lane using 10% SDS-PAGE, followed by transfer to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and chemiluminescent substrate were used for visualization.