DTD1 Antibody / D-aminoacyl-tRNA deacylase 1 Antibody (FY13598)

DTD1 Antibody by IF in A549 cells. Immunofluorescence staining using D-aminoacyl-tRNA deacylase 1 Antibody shows green cytoplasmic and perinuclear staining, with green fluorescence also overlapping portions of the nuclear area. Nuclei are counterstained blue with DAPI. Cells underwent enzyme-mediated antigen retrieval for 15 minutes and blocking with 10% goat serum. Primary antibody was applied at 5 ug/mL overnight at 4oC, followed by a Fluoro488-conjugated secondary antibody at 1:500 for 30 minutes at 37oC. The observed staining distribution is described without assuming organelle-specific colocalization.

DTD1 Antibody by WB in human and rodent samples. Western blot analysis using D-aminoacyl-tRNA deacylase 1 Antibody detects a prominent immunoreactive band near 24 kDa in human MCF-7 (lane 1), A549 (lane 2), rat brain (lane 3), and mouse brain (lane 4). The calculated molecular weight is approximately 23 kDa. Reduced samples containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody at 1:5000 and a chemiluminescent substrate. The band positions are described relative to the molecular weight standards in the blot.