• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> DSG3 Antibody for IHC / Desmoglein 3

DSG3 Antibody for IHC / Desmoglein 3 [clone MSVA-543M] (V6069)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V6069-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6069-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Bulk quote request
Immunohistochemistry validation of DSG3 Antibody for IHC clone MSVA-543M across a broad panel of normal and malignant human tissues. Formalin-fixed, paraffin-embedded tissue microarrays demonstrate membranous staining in stratified squamous epithelia, including skin, esophagus, and other mucosal tissues, consistent with Desmoglein 3 localization at desmosomal cell-cell junctions. Most non-squamous tissues show minimal to absent staining, while squamous cell carcinomas display preserved membranous expression patterns. Overall staining distribution across normal and cancer tissues is consistent with publicly available expression data, including findings reported in the Human Protein Atlas, supporting the expected tissue-specific expression profile of DSG3.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MSVA-543M
UniProt P32926
Localization Cell junction, Cell membrane, Desmosome
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This DSG3/Desmoglein 3 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

DSG3 Antibody for IHC clone MSVA-543M recognizes Desmoglein 3, a calcium-dependent desmosomal cadherin encoded by the DSG3 gene on chromosome 18q12.1. Desmoglein 3, commonly referred to as DSG3 in the literature, is a single-pass transmembrane glycoprotein localized to desmosomes at the plasma membrane of stratified epithelial cells where it mediates strong intercellular adhesion. DSG3 antibody, also known as Desmoglein 3 antibody, is widely used in studies of epithelial differentiation, mucocutaneous integrity, and squamous cell carcinoma biology. This mouse monoclonal antibody supports detection of membranous DSG3 expression in formalin-fixed, paraffin-embedded tissue sections.

Desmoglein 3 is a member of the cadherin superfamily and functions as a core structural component of desmosomal junctions. Its extracellular cadherin repeats enable calcium-dependent homophilic adhesion between adjacent epithelial cells, while its intracellular domain associates with desmosomal plaque proteins including Plakoglobin and Desmoplakin. Through these interactions, Desmoglein 3 anchors keratin intermediate filaments to the plasma membrane, reinforcing epithelial cohesion in tissues subjected to mechanical stress such as skin and mucosal surfaces.

Expression of DSG3 is most prominent in the basal and immediate suprabasal layers of stratified squamous epithelia, including oral mucosa, esophagus, cervix, and epidermis. In contrast to Desmoglein 1, which is enriched in superficial epidermal layers, Desmoglein 3 is concentrated in deeper epithelial layers and plays a central role in maintaining mucocutaneous stability. Autoantibodies directed against Desmoglein 3 are strongly associated with pemphigus vulgaris, where disruption of desmosomal adhesion results in intraepidermal blister formation. Altered DSG3 expression has also been reported in squamous cell carcinoma and other epithelial malignancies, where desmosomal remodeling may influence tumor differentiation and invasion patterns.

Structurally, Desmoglein 3 contains multiple extracellular cadherin domains, a single transmembrane segment, and an intracellular tail that integrates into the desmosomal plaque complex. Beyond its adhesive function, DSG3 participates in signaling pathways regulating keratinocyte proliferation, differentiation, and epithelial homeostasis. Through its established role in desmosomal architecture and epithelial integrity, Desmoglein 3 remains an important marker in epithelial and oncologic research.

Application Notes

1. Optimal dilution of the DSG3 antibody for IHC should be determined by the researcher.

2. This DSG3/Desmoglein 3 antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

A recombinant fragment (around amino acids 379-491) human DSG3 protein (exact sequence is proprietary) was used as the immunogen for the DSG3 antibody for IHC.

Storage

DSG3/Desmoglein 3 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

Related Products

   1.   View all DSG3/Desmoglein 3 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.