• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Recombinant DSG3 Antibody rDSG3/8611 / Desmoglein 3

Recombinant DSG3 Antibody rDSG3/8611 / Desmoglein 3 [clone rDSG3/8611] (V4569)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V4569-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
Image
V4569-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
Image
V4569SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recmousemono
Bulk quote request
›
Immunohistochemistry of Recombinant DSG3 Antibody rDSG3/8611 in human skin tissue. Formalin-fixed, paraffin-embedded human skin demonstrates strong membranous staining of basal and suprabasal keratinocytes, consistent with Desmoglein 3 localization at desmosomal cell-cell junctions within stratified squamous epithelium. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining. The recombinant monoclonal antibody clone rDSG3/8611 was used as the primary antibody. Inset: PBS was used in place of the primary antibody as a secondary antibody negative control.
SDS-PAGE analysis of purified, BSA-free recombinant DSG3 antibody rDSG3/8611 as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rDSG3/8611
Purity Protein A/G affinity
UniProt P32926
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This DSG3 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

Recombinant DSG3 Antibody rDSG3/8611 recognizes Desmoglein 3, a calcium-dependent desmosomal cadherin encoded by the DSG3 gene on chromosome 18q12.1. Desmoglein 3, also referred to as DSG3 in the literature, is a single-pass transmembrane glycoprotein localized to desmosomes at the plasma membrane of stratified epithelial cells, where it mediates strong intercellular adhesion and supports epithelial structural integrity. DSG3 antibody, also known as Desmoglein 3 antibody, is widely used in research focused on epithelial biology, mucosal differentiation, and autoimmune blistering diseases. This recombinant monoclonal antibody clone rDSG3/8611 supports detection of membranous DSG3 expression in epithelial tissues.

Desmoglein 3 is a member of the cadherin superfamily and functions as a core component of desmosomal junctions. Its extracellular cadherin repeats mediate calcium-dependent homophilic adhesion between adjacent epithelial cells, while its cytoplasmic domain associates with desmosomal plaque proteins including Plakoglobin and Desmoplakin. Through these interactions, Desmoglein 3 anchors keratin intermediate filaments to the plasma membrane, reinforcing mechanical stability in tissues exposed to friction and shear stress, particularly mucosal epithelia and basal epidermal layers.

Expression of DSG3 is predominantly observed in the basal and suprabasal layers of stratified squamous epithelia, including oral mucosa, esophagus, cervix, and skin. In contrast to Desmoglein 1, which is enriched in superficial epidermal layers, Desmoglein 3 is more abundant in deeper epithelial layers and plays a critical role in maintaining mucocutaneous adhesion. Autoantibodies directed against Desmoglein 3 are strongly associated with pemphigus vulgaris, where disruption of desmosomal adhesion leads to intraepidermal blister formation. Altered DSG3 expression has also been reported in squamous cell carcinomas and other epithelial malignancies.

Structurally, Desmoglein 3 contains multiple extracellular cadherin domains, a transmembrane region, and an intracellular tail that anchors to the desmosomal plaque complex. Beyond structural adhesion, DSG3 participates in signaling pathways that influence keratinocyte proliferation, differentiation, and epithelial homeostasis. Dysregulated expression may contribute to altered epithelial architecture and tumor progression in stratified epithelia. Through its essential role in desmosomal cohesion, Desmoglein 3 remains central to research in epithelial adhesion biology and mucocutaneous disease mechanisms.

Application Notes

Optimal dilution of the recombinant DSG3 antibody rDSG3/8611 should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 300-500) from the human protein was used as the immunogen for the recombinant DSG3 antibody rDSG3/8611.

Storage

Aliquot the DSG3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all DSG3 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.