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Home >> Antibodies >> DSG3 Antibody / Desmoglein 3

DSG3 Antibody / Desmoglein 3 [clone MSVA-543M] (V6069)

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  Catalog No Formulation Size Price (USD)  
Image V6069-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6069-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Desmoglein-3 Mouse Monoclonal Antibody (MSVA-543M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MSVA-543M
UniProt P32926
Localization Cell junction, Cell membrane, Desmosome
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This DSG3/Desmoglein 3 antibody is available for research use only.
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Application Notes

1. Optimal dilution of the DSG3/Desmoglein 3 antibody should be determined by the researcher.

2. This DSG3/Desmoglein 3 antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment (around amino acids 379-491) human DSG3 protein (exact sequence is proprietary) was used as the immuongen for the DSG3/Desmoglein 3 antibody.

Storage

DSG3/Desmoglein 3 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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