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Home >> Antibodies >> DSG1 Antibody for IHC / Desmoglein 1

DSG1 Antibody for IHC / Desmoglein 1 [clone MSVA-544M] (V6068)

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  Catalog No Formulation Size Price (USD)  
Image V6068-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
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V6068-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Immunohistochemistry of DSG1 Antibody for IHC in normal and cancer tissue microarrays. Formalin-fixed, paraffin-embedded human tissue microarrays were stained with recombinant Desmoglein-1 mouse monoclonal antibody clone MSVA-544M. In normal tissues, strong membranous staining is observed in stratified squamous epithelium including skin, esophagus, and tonsil surface epithelium, consistent with Desmoglein 1 localization in suprabasal keratinocytes. Most non-stratified epithelia and non-epithelial tissues show little to no staining.
In the cancer tissue panel, membranous staining is present in squamous cell carcinomas, including larynx, oral cavity, vulva, and cervix, as well as in selected urothelial carcinomas, consistent with retained epithelial differentiation. Most adenocarcinomas and non-epithelial malignancies demonstrate minimal or negative staining.
The observed tissue distribution and tumor staining profile align with established Desmoglein 1 expression patterns reported in the Human Protein Atlas, supporting the specificity of clone MSVA-544M for desmosomal epithelial cell-cell junctions.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name MSVA-544M
UniProt Q02413
Localization Cell junction, Cell membrane, Desmosome
Applications Immunohistochemistry (FFPE) : 1:75-1:150
Limitations This DSG1/Desmoglein 1 antibody is available for research use only.
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Description

DSG1 Antibody for IHC clone MSVA-544M recognizes Desmoglein 1, a calcium-dependent desmosomal cadherin encoded by the DSG1 gene on chromosome 18q12.1. Desmoglein 1, also referred to as DSG1 in the literature, is a single-pass transmembrane glycoprotein localized to desmosomes at the plasma membrane of stratified epithelial cells, where it mediates strong intercellular adhesion and preserves epidermal structural integrity. DSG1 antibody, also known as Desmoglein 1 antibody, is widely used in research focused on epithelial differentiation, barrier formation, and autoimmune blistering diseases. This mouse monoclonal antibody clone MSVA-544M is designed to support immunohistochemical detection of membranous DSG1 expression in tissue sections.

Desmoglein 1 is a member of the cadherin superfamily and functions as a core structural component of desmosomal junctions. Its extracellular cadherin repeats mediate calcium-dependent homophilic adhesion between adjacent keratinocytes, while its cytoplasmic domain associates with desmosomal plaque proteins including Plakoglobin and Desmoplakin. Through these interactions, Desmoglein 1 anchors keratin intermediate filaments to the plasma membrane, reinforcing mechanical resilience in tissues exposed to friction and physical stress, particularly the epidermis and mucosal epithelium.

Expression of DSG1 is predominantly observed in the suprabasal layers of stratified squamous epithelia, including skin, oral mucosa, and esophagus. It is highly expressed in differentiated keratinocytes and contributes to epidermal stratification and barrier function. Autoantibodies directed against Desmoglein 1 are implicated in pemphigus foliaceus, where disruption of desmosomal adhesion results in superficial epidermal blistering. Altered DSG1 expression has also been associated with inherited skin disorders characterized by impaired epidermal cohesion and inflammatory phenotypes.

Structurally, Desmoglein 1 contains multiple extracellular cadherin domains, a transmembrane region, and an intracellular tail that connects to the desmosomal plaque complex. Beyond structural adhesion, Desmoglein 1 participates in signaling pathways influencing keratinocyte differentiation and epidermal homeostasis. Dysregulated DSG1 expression may alter epithelial architecture and contribute to inflammatory or neoplastic processes in stratified epithelia. Through its essential role in maintaining desmosomal integrity, Desmoglein 1 remains central to research in epithelial biology and dermatologic disease mechanisms.

Application Notes

1. Optimal dilution of the DSG1/Desmoglein 1 antibody should be determined by the researcher.

2. This DSG1 antibody for IHC is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis was used as the immunogen for the DSG1 antibody for IHC.

Storage

DSG1/Desmoglein 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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