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Home >> Antibodies >> dsDNA Antibody / Double Stranded DNA

dsDNA Antibody / Double Stranded DNA [clone DSD/8204R] (V5321)

  Catalog No Formulation Size Price (USD)  
Image V5321-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5321-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5321SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human placental tissue with dsDNA antibody (clone DSD/8204R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer (serous) tissue with dsDNA antibody (clone DSD/8204R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name DSD/8204R
Purity Protein A affinity
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This dsDNA antibody is available for research use only.
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Description

This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Application Notes

Optimal dilution of the dsDNA antibody should be determined by the researcher.

Immunogen

Burkitt's cell nuclei were used as the immunogen for the dsDNA antibody.

Storage

Aliquot the dsDNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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