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Home >> Antibodies >> dsDNA Antibody

dsDNA Antibody [clone 121-3] (V3075)

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  Catalog No Formulation Size Price (USD)  
Image V3075-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3075-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3075SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3075IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human colon carcinoma with dsDNA antibody (clone 121-3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human colon carcinoma with dsDNA antibody (clone 121-3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with dsDNA antibody (clone 121-3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Flow cytometry testing of fixed and permeabilized human Jurkat cells with dsDNA antibody (clone 121-3); Red=isotype control, Blue= dsDNA antibody.
SDS-PAGE analysis of purified, BSA-free dsDNA antibody (clone 121-3) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG3, kappa
Clone Name 121-3
Purity Protein G affinity chromatography
Localization Nuclear
Applications Flow cytometry : 1-2ug/million cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This dsDNA antibody is available for research use only.
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Description

Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.

Application Notes

Optimal dilution of the dsDNA antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.

Storage

Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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