dsDNA Antibody / Double Stranded DNA [clone 121-3] (V3075)

Description

dsDNA antibody clone 121-3 is a monoclonal antibody that detects double-stranded DNA, a critical biomolecule that encodes genetic information in all living cells. Antibodies against dsDNA are particularly important in autoimmune disease research, as they represent hallmark autoantibodies in systemic lupus erythematosus. This reagent is also widely used in molecular biology to evaluate DNA integrity and monitor chromatin-related processes. NSJ Bioreagents supplies this antibody for immunology, autoimmunity, and cell biology research.

The antibody produces strong nuclear staining in cells, reflecting the distribution of chromosomal DNA. In immunology, dsDNA detection supports research into autoimmune pathogenesis. Anti-dsDNA antibodies are a hallmark of systemic lupus erythematosus and are used to monitor disease activity. Clone 121-3 provides researchers with a consistent tool to evaluate DNA autoantigen recognition and immune system dysregulation.

In cell biology, dsDNA antibody clone 121-3 has been employed to monitor DNA fragmentation during apoptosis. Detection of fragmented chromosomal DNA provides insight into programmed cell death pathways and their regulation in health and disease. The antibody has also been used in models of DNA damage repair, enabling exploration of genomic stability and checkpoint activation.

In oncology, dsDNA antibodies are applied to assess DNA damage in cancer cells subjected to genotoxic stress. Monitoring DNA integrity provides valuable information about tumor sensitivity to radiation and chemotherapy. This antibody supports translational studies that aim to optimize therapeutic strategies by linking DNA damage with treatment response.

In molecular biology, the antibody has been used in studies of DNA organization, chromatin remodeling, and replication. Its specificity for double-stranded DNA provides reliable detection of genomic material in diverse experimental contexts.

Validated in multiple systems, the antibody consistently produces specific nuclear staining with minimal background. Alternate names include double-stranded DNA antibody, lupus biomarker antibody, and chromosomal DNA antibody.

Application Notes

Optimal dilution of the dsDNA antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.

Storage

Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).