DPYD Antibody / DPD Antibody (RQ4203)

DPYD Antibody Human Cervical Cancer Cell IF. Immunofluorescent staining of SiHa cells using DPYD antibody demonstrated predominantly cytoplasmic fluorescence, consistent with the localization of dihydropyrimidine dehydrogenase as a cytosolic enzyme responsible for pyrimidine and fluoropyrimidine metabolism. Cells were treated with enzyme antigen retrieval reagent for 15 minutes, blocked with 10% goat serum, and incubated with the primary antibody (5 ug/ml) overnight at 4°C. Cy3-conjugated goat anti-rabbit IgG was used as the secondary antibody, and nuclei were counterstained with DAPI (blue). This DPYD Antibody, also called a DPD Antibody, is useful for investigating pyrimidine catabolism, fluoropyrimidine metabolism, chemotherapy response, and cancer pharmacology.

DPYD Antibody Human, Mouse and Rat Liver WB. Western blot testing of human SKOV3 (lane 1), U251 (lane 2), THP-1 (lane 3), rat liver (lane 4), and mouse liver (lane 5) lysates using DPYD antibody detected a specific band at approximately 111 kDa, consistent with the expected molecular weight of dihydropyrimidine dehydrogenase. The strongest signals were observed in rat and mouse liver, in agreement with the well-established high hepatic expression of DPYD, the rate-limiting enzyme responsible for pyrimidine and fluoropyrimidine catabolism. This DPYD Antibody, also called a DPD Antibody, is useful for investigating pyrimidine metabolism, fluoropyrimidine metabolism, chemotherapy response, and cancer pharmacology.