DPYD Antibody / Pyrimidine Metabolism Enzyme Antibody (R30854)

DPYD Antibody HepG2 Cells FACS. Flow cytometric analysis of fixed and permeabilized human HepG2 cells was performed using DPYD antibody at 1 ug per million cells following blocking with goat sera. DPYD, also known as Dihydropyrimidine Dehydrogenase and a Pyrimidine Metabolism Enzyme, catalyzes the rate limiting step in pyrimidine catabolism and plays a critical role in fluoropyrimidine drug metabolism. Red histogram: cells alone. Green histogram: isotype control. Blue histogram: DPYD antibody. The pronounced rightward shift of the DPYD antibody signal relative to the controls demonstrates specific detection of endogenous DPYD expression in HepG2 cells. These results support the utility of DPYD Antibody for studies of nucleotide metabolism, pharmacogenomics, and cancer biology.

DPYD Antibody Human Mouse Rat WB. Western blot analysis of DPYD expression using a DPYD antibody. Lane 1: human HeLa whole cell lysate. Lane 2: human THP-1 whole cell lysate. Lane 3: rat liver tissue lysate. Lane 4: mouse liver tissue lysate. DPYD, also known as Dihydropyrimidine Dehydrogenase and a Pyrimidine Metabolism Enzyme, catalyzes the rate limiting step in pyrimidine catabolism and is essential for fluoropyrimidine drug metabolism. A distinct band is detected at approximately 111 kDa, corresponding to the expected molecular weight of full length DPYD. Signal intensity is stronger in rat and mouse liver samples, consistent with the prominent role of hepatic tissues in nucleotide turnover and drug metabolism. These results support the utility of DPYD Antibody for studies of pyrimidine metabolism, pharmacogenomics, and metabolic regulation.