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Home >> Antibodies >> DPT Antibody / Dermatopontin

DPT Antibody / Dermatopontin (F54932)

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  Catalog No Formulation Size Price (USD)  
Image F54932-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
Image
F54932-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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IHC testing of FFPE human brain tissue with DPT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human brain tissue with DPT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of FFPE human brain tissue with DPT antibody (green) and propidium iodide (red).
Western blot testing of human HL60 cell lysate with DPT antibody. Expected molecular weight: 22-24 kDa.
Flow cytometry testing of human NCI-H292 cells with DPT antibody; Blue=isotype control, Green= DPT antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q07507
Applications Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Western Blot : 1:500-1:1000
Limitations This DPT antibody is available for research use only.
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Description

DPT is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. This protein is found in various tissues and many of its tyrosine residues are sulphated. The protein is postulated to modify the behavior of TGF-beta through interaction with decorin.

Application Notes

The stated application concentrations are suggested starting points. Titration of the DPT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 102-128 from the human protein was used as the immunogen for the DPT antibody.

Storage

Aliquot the DPT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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