DPF2 Antibody / Zinc finger protein ubi-d4 Antibody (FY13595)

DPF2 Antibody by IF in HeLa cells. Immunofluorescence staining using Zinc finger protein ubi-d4 Antibody shows bright green nuclear DPF2 signal with darker nucleolar regions in HeLa cells. Red alpha-tubulin staining delineates the cytoplasmic microtubule network. The nuclear enrichment is consistent with the transcriptional regulatory role of DPF2. Cells underwent enzyme-mediated antigen retrieval, blocking with 10% goat serum, and overnight incubation at 4oC with DPF2 and alpha-tubulin primary antibodies. Fluoro488 and Cy3 secondary antibodies were applied at 1:500 for 30 minutes at 37oC.

DPF2 Antibody by IHC in human lung carcinoma. Immunohistochemistry of paraffin-embedded human lung carcinoma using Zinc finger protein ubi-d4 Antibody shows brown staining in epithelial tumor cells, including regions surrounding glandular spaces. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection. Hematoxylin provides the blue nuclear counterstain.

DPF2 Antibody by WB in human and rodent samples. Western blot analysis using Zinc finger protein ubi-d4 Antibody detects an immunoreactive band near 40 kDa in human Jurkat (lane 1), HeLa (lane 2), Y79 (lane 3), HepG2 (lane 4), rat thymus (lane 5), and mouse thymus (lane 6). The calculated molecular weight is approximately 44.2 kDa. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

DPF2 Antibody by IHC in human breast carcinoma. Immunohistochemistry of paraffin-embedded human breast carcinoma using Zinc finger protein ubi-d4 Antibody shows brown staining in tumor cells bordering glandular and duct-like spaces. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection. Hematoxylin provides the blue nuclear counterstain.