DOCK4 Antibody / Cell Migration Protein Antibody (RQ7372)

DOCK4 Antibody Human Skeletal Muscle IHC. Immunohistochemistry staining of FFPE human skeletal muscle using DOCK4 antibody demonstrates widespread cytoplasmic staining throughout skeletal muscle fibers, consistent with expression of Dedicator of Cytokinesis 4 (DOCK4). DOCK4 is a cell migration protein that regulates Rac-family GTPase signaling pathways involved in cytoskeletal organization, cellular adhesion, and maintenance of tissue architecture. The observed staining pattern within mature muscle fibers is consistent with the role of DOCK4 in coordinating cytoskeletal dynamics and cellular organization. These findings support the utility of DOCK4 antibody for studies of cell migration, cytoskeletal regulation, and tissue morphogenesis pathways. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

DOCK4 Antibody Human and Rat WB. Western blot analysis of human 293T and rat L6 cell lysates using DOCK4 antibody demonstrates a distinct immunoreactive band at approximately 225 kDa, consistent with the expected molecular weight of Dedicator of Cytokinesis 4 (DOCK4). DOCK4 is a cell migration protein that functions as a guanine nucleotide exchange factor regulating Rac-family GTPase signaling, cytoskeletal remodeling, and cell adhesion pathways. The observed expression in both human and rat samples supports the utility of this antibody for investigating cellular motility, tissue organization, cytoskeletal regulation, and developmental signaling mechanisms. Predicted molecular weight: ~225 kDa.

DOCK4 Antibody SiHa Flow Cytometry. Flow cytometric analysis of human SiHa cells using DOCK4 antibody demonstrates a clear rightward shift of the blue histogram (DOCK4 antibody) relative to the green histogram (isotype control) and red histogram (cells alone), consistent with expression of Dedicator of Cytokinesis 4 (DOCK4). DOCK4 is a cell migration protein that regulates Rac-family GTPase signaling pathways involved in cytoskeletal organization, cell adhesion, and directed cellular movement. The observed staining pattern supports detection of endogenous DOCK4 expression and demonstrates the utility of this antibody for studies of cellular motility, cytoskeletal regulation, tissue morphogenesis, and signaling pathways governing cell-cell interactions. Cells were stained with 1 ug antibody per million cells following blocking with goat sera.