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Home >> Antibodies >> DNTT Antibody / Terminal deoxynucleotidyl transferase

DNTT Antibody / Terminal deoxynucleotidyl transferase [clone MSVA-453R] (V6067)

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  Catalog No Formulation Size Price (USD)  
Image V6067-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6067-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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DNA nucleotidylexotransferase Rabbit Recombinant Monoclonal Antibody (MSVA-453R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas. <br><br>Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
DNA nucleotidylexotransferase Rabbit Recombinant Monoclonal Antibody (MSVA-453R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-453R
UniProt P04053
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This DNTT/Terminal deoxynucleotidyl transferase antibody is available for research use only.
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Application Notes

1. Optimal dilution of the DNTT/Terminal deoxynucleotidyl transferase antibody should be determined by the researcher.

2. This DNTT/Terminal deoxynucleotidyl transferase antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment (around amino acids 52-192) of human DNTT protein (exact sequence is proprietary) was used as the immuongen for the DNTT/Terminal deoxynucleotidyl transferase antibody.

Storage

DNTT/Terminal deoxynucleotidyl transferase antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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