- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
DNTT Antibody Mouse Recombinant MAb / TdT / DNA Nucleotidylexotransferase [clone rDNTT/6909] (V9490)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V9490-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V9490-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V9490SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rDNTT/6909 |
| Purity | Protein A/G affinity |
| UniProt | P04053 |
| Localization | Nucleus |
| Applications | ELISA (Use BSA-free Format For Coating) : Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This DNTT antibody is available for research use only. |
|
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DNTT antibody, also known as Terminal deoxynucleotidyl transferase antibody, recognizes a specialized DNA polymerase commonly referred to as TdT. The DNTT Antibody Mouse Recombinant MAb Clone rDNTT/6909 targets Terminal deoxynucleotidyl transferase, a nuclear enzyme encoded by the human DNTT gene located on chromosome 10q23-q24. TdT is a member of the DNA polymerase X family and is primarily localized to the nucleus of immature lymphoid cells, where it participates in antigen receptor gene rearrangement. DNTT antibody, also referred to as TdT antibody and Terminal deoxynucleotidyl transferase antibody in the literature, is widely used in research focused on lymphoid development and hematologic malignancies.
Terminal deoxynucleotidyl transferase catalyzes the template-independent addition of deoxynucleotides to the 3-prime hydroxyl termini of DNA. This unique activity is critical during V(D)J recombination in developing B and T lymphocytes, where TdT introduces N-nucleotide additions that significantly enhance antigen receptor diversity. The enzyme contains conserved polymerase domains characteristic of the Pol X family and interacts with components of the recombination machinery, including RAG1 and RAG2 complexes within nuclear recombination centers. A DNTT antibody is therefore valuable for studying early lymphopoiesis, particularly in bone marrow and thymic progenitor populations.
Expression of DNTT is developmentally regulated and largely restricted to immature, pre-B and pre-T lymphoblasts. It is minimally expressed in mature peripheral lymphocytes, making it a well-established marker of lymphoid immaturity. High levels of TdT expression are commonly observed in acute lymphoblastic leukemia and certain lymphoblastic lymphomas, where DNTT antibody detection supports diagnostic and mechanistic investigations. In normal physiology, TdT expression peaks during early lymphoid differentiation and declines as cells undergo maturation and antigen receptor selection.
Structurally, TdT contains a catalytic core domain responsible for nucleotide transfer and regulatory regions that influence substrate selection and protein interactions. Alternative splicing of DNTT can generate isoforms with distinct biochemical properties, potentially influencing nucleotide incorporation efficiency and cellular function. The enzyme operates within the non-homologous end joining pathway, contributing to junctional diversity without requiring a DNA template. Through its central role in adaptive immune repertoire formation, TdT supports immune competence while also contributing to genetic variability that underlies lymphoid neoplasia when dysregulated.
The mouse recombinant monoclonal DNTT antibody clone rDNTT/6909 is suitable for detecting TdT expression in research applications focused on lymphoid differentiation, immune system development, and leukemia biology. This DNTT antibody supports studies examining nuclear localization patterns and the biology of early hematopoietic progenitors.
Optimal dilution of the DNTT antibody mouse recombinant mAb should be determined by the researcher.
A portion of amino acids 1-100 was used as the immunogen for the DNTT antibody mouse recombinant mAb.
Aliquot the DNTT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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