DNMT1 Antibody / DNA Methylation Maintenance Enzyme Antibody (RQ4002)

DNMT1 Antibody U-2 OS Cell IF. Immunofluorescence analysis of FFPE human U-2 OS cells using DNMT1 Antibody (green) shows predominant nuclear staining consistent with localization of DNA methyltransferase 1 during epigenetic maintenance. Alpha Tubulin mAb (red) highlights the cytoskeletal network, providing cellular context and contrast. The nuclear enrichment of signal supports DNMT1 function in DNA methylation and chromatin regulation. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min.

DNMT1 Antibody Multi-Cell Line WB. Western blot analysis of human 1) HeLa, 2) 293T, and 3) SH-SY5Y cell lysates using DNMT1 Antibody detects a band at approximately 180–200 kDa, consistent with the predicted molecular weight of DNMT1 / DNA methyltransferase 1. The strong, high-molecular-weight signal across all three cell lines supports detection of this nuclear maintenance methyltransferase, aligning with its role in preserving DNA methylation patterns during cell proliferation.

DNMT1 Antibody 293T Cell FACS. Flow cytometry analysis of fixed and permeabilized human 293T cells using DNMT1 Antibody (blue) shows a clear rightward shift relative to unstained cells (red) and isotype control (green), indicating detection of DNMT1 / DNA methyltransferase 1 expression. The intracellular signal distribution is consistent with localization of this nuclear epigenetic regulator involved in maintenance of DNA methylation during cell proliferation.

DNMT1 Antibody Breast Cancer Tissue IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using DNMT1 Antibody shows nuclear HRP-DAB brown staining in tumor epithelial cells, consistent with localization of DNMT1 / DNA methyltransferase 1 as a nuclear epigenetic regulator. Staining intensity varies across tumor regions, with positive nuclei distributed within malignant cell populations, while surrounding stromal elements display lower signal. Hematoxylin counterstain provides nuclear contrast and tissue architecture. HIER: boil FFPE tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

DNMT1 Antibody Pancreatic Cancer Tissue IHC. Immunohistochemistry analysis of FFPE human pancreatic carcinoma tissue using DNMT1 Antibody shows nuclear HRP-DAB brown staining in tumor epithelial cells, consistent with localization of DNMT1 / DNA methyltransferase 1 as a nuclear epigenetic regulator. Positive nuclei are observed within malignant glandular structures, while surrounding stromal regions display lower staining intensity. Hematoxylin counterstain provides nuclear contrast and tissue architecture. HIER: boil FFPE tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.