DNER Antibody / Delta/notch like EGF repeat containing Antibody (FY13579)

DNER Antibody by IHC in human cerebellum. Immunohistochemistry of paraffin-embedded human cerebellum using Delta/notch like EGF repeat containing Antibody shows brown neuropil staining in the pale molecular layer beneath the densely hematoxylin-stained granule cell layer, with labeling around the Purkinje cell region. Nuclei are counterstained blue with hematoxylin. Sections underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0, blocking with 10% goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Detection used a peroxidase-conjugated secondary antibody and DAB chromogen.

DNER Antibody by FACS in fixed HeLa cells. Flow cytometry using Delta/notch like EGF repeat containing Antibody shows a rightward fluorescence shift of the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and unstained blank control (red). Cells were fixed with 4% paraformaldehyde, blocked with normal goat serum, and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. A Fluoro488-conjugated secondary antibody was used for detection. This experiment was performed on fixed cells and does not demonstrate live-cell surface staining.

DNER Antibody by WB in rodent brain. Western blot analysis using Delta/notch like EGF repeat containing Antibody detects a band near 130 kDa in rat brain (lane 1) and mouse brain (lane 2), compared with a calculated molecular weight of approximately 78 kDa. The higher apparent molecular weight is not assigned to a specific modified form in this experiment. Reduced lysates containing 30 ug total protein per lane were resolved by 8% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk, followed by primary antibody incubation at 0.5 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and chemiluminescent substrate.

DNER Antibody by IHC in human cerebellum. Immunohistochemistry of paraffin-embedded human cerebellum using Delta/notch like EGF repeat containing Antibody shows brown staining throughout the molecular layer, adjacent to the densely packed blue granule cell layer. Nuclei are counterstained blue with hematoxylin. Sections underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0, blocking with 10% goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Detection used a peroxidase-conjugated secondary antibody and DAB chromogen.

DNER Antibody by IHC in rat brain. Immunohistochemistry of paraffin-embedded rat brain using Delta/notch like EGF repeat containing Antibody shows brown staining in scattered neuronal cell bodies and surrounding neuropil. Nuclei are counterstained blue with hematoxylin. Sections underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0, blocking with 10% goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Detection used a peroxidase-conjugated secondary antibody and DAB chromogen.