DNASE2 Antibody / Deoxyribonuclease-2-alpha Antibody (FY13609)

DNASE2 Antibody by IHC in human prostate carcinoma. Immunohistochemistry of paraffin-embedded human prostate carcinoma using Deoxyribonuclease-2-alpha Antibody shows strong brown cytoplasmic staining in glandular epithelial tumor cells. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Immunoreactivity was visualized with an HRP-conjugated secondary antibody and DAB chromogen; hematoxylin counterstaining identifies tissue nuclei.

DNASE2 Antibody by WB in HeLa cells. Western blot analysis using Deoxyribonuclease-2-alpha Antibody detects a band near 32 kDa in human HeLa whole-cell lysate (lane 1). The observed migration is below the calculated molecular weight of approximately 40 kDa. Published studies have reported DNASE2 near 32 kDa in human tissues and proteolytically processed DNase II forms near 30 kDa in other mammals, which may account for the lower apparent mass. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and chemiluminescence.