DNASE1L3 Antibody / Deoxyribonuclease gamma Antibody (FY13584)

DNASE1L3 Antibody by IF in HaCaT cells. Immunofluorescence using Deoxyribonuclease gamma Antibody shows green nuclear and perinuclear signal in HaCaT keratinocytes, with red alpha-tubulin staining delineating the cytoplasmic microtubule network. The apparent nuclear enrichment is descriptive and does not establish the localization of the secreted mature protein. Cells underwent enzyme-mediated antigen retrieval for 15 minutes and blocking with 10% goat serum. Primary antibodies were incubated overnight at 4oC at 5 ug/mL for DNASE1L3, followed by Fluoro488 and Cy3 secondary antibodies at 1:500 for 30 minutes at 37oC.

DNASE1L3 Antibody by IHC in rat spleen tissue. Immunohistochemistry of paraffin-embedded rat spleen tissue using Deoxyribonuclease gamma Antibody shows brown staining in scattered splenic cells amid densely packed blue-stained lymphoid nuclei. Nuclei are counterstained blue with hematoxylin. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by a peroxidase-conjugated secondary antibody and DAB chromogenic detection.

DNASE1L3 Antibody by WB in human cell lines and rodent tissues. Western blot analysis using Deoxyribonuclease gamma Antibody detects a band near 36 kDa in human 293T (lane 1), Daudi (lane 2), A431 (lane 3), rat liver (lane 4), and mouse spleen (lane 5). The calculated molecular weight is approximately 35.5 kDa. Reduced samples containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.