DNAJB5 Antibody / DnaJ homolog subfamily B member 5 Antibody (FY13663)

DNAJB5 Antibody by FCM in Fixed SH-SY5Y Cells. Flow cytometry analysis of fixed, permeabilized SH-SY5Y cells using DNAJB5 Antibody shows a blue test histogram shifted toward higher fluorescence relative to the green isotype control; the red histogram is the blank. The assay detects intracellular antibody labeling rather than live-cell surface staining.

DNAJB5 Antibody by IHC in Human Colon Cancer Tissue. Immunohistochemistry of paraffin-embedded human colon cancer tissue using DNAJB5 Antibody shows widespread brown DAB immunoreactivity in tumor cells, including conspicuous nuclear-associated staining. Nuclei are counterstained blue with hematoxylin and staining intensity varies across tumor glands.

DNAJB5 Antibody by IHC in Mouse Brain Tissue. Immunohistochemistry of paraffin-embedded mouse brain tissue using DNAJB5 Antibody shows scattered dark nuclear-associated staining against a relatively pale neuropil background. Blue hematoxylin counterstaining outlines other nuclei; labeling is not uniform across the section.

DNAJB5 Antibody by IHC in Rat Brain Tissue. Immunohistochemistry of paraffin-embedded rat brain tissue using DNAJB5 Antibody shows variable brown DAB immunoreactivity in cells distributed throughout the neuropil. Hematoxylin counterstaining highlights numerous nuclei and areas of relatively weak background staining.

DNAJB5 Antibody by IHC in Rat Brain Tissue. Immunohistochemistry analysis of DNAJB5 expression in a paraffin-embedded rat brain section using DNAJB5 Antibody. Brown DAB immunoreactivity varies across the layered brain tissue, with hematoxylin highlighting densely distributed nuclei. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The section was incubated with primary antibody at 2 ug/ml overnight at 4oC and detected with a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen. The tissue identification is confirmed in the supplier datasheet for A13716-1.

DNAJB5 Antibody by IF in U2OS Cells. Immunofluorescence microscopy of U2OS cells labeled with DNAJB5 Antibody shows predominantly cytoplasmic red fluorescence with a granular distribution around green counterstained nuclei. The staining is present across multiple cells and does not independently establish an organelle-specific location.

DNAJB5 Antibody by WB in Human and Rodent Lysates. Western blot analysis using DNAJB5 Antibody demonstrates a principal band near 38-40 kDa across eight lysates. Lanes 1-4 represent SH-SY5Y, U251, Jurkat, and PC-3 human cell lines; lanes 5-6 represent rat brain and C6 cells; lanes 7-8 represent mouse brain and Neuro-2a cells. The predominant band is near the expected size of DNAJB5, with some variation in intensity between lanes. Additional lower-migrating bands are apparent in the mouse samples, but their molecular identities cannot be determined from the image alone. The results show antibody reactivity in the examined human and rodent preparations.