DNAJB4 Antibody / DnaJ homolog subfamily B member 4 Antibody (FY13587)

DNAJB4 Antibody by FACS in A549 cells. Flow cytometry analysis using DnaJ homolog subfamily B member 4 Antibody shows a rightward shift of the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and unstained blank (red). A549 cells were fixed with 4% paraformaldehyde. Primary antibody was applied at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by a fluorescent anti-rabbit IgG secondary antibody. The histogram demonstrates detectable antibody-associated fluorescence under these assay conditions.

DNAJB4 Antibody by WB in human cell lines and rodent brain. Western blot analysis using DnaJ homolog subfamily B member 4 Antibody detects a prominent band near 38 kDa, compared with the calculated molecular weight of approximately 38 kDa. Lanes 1-4 contain HeLa, K562, A549 and U2OS whole-cell lysates, respectively; lanes 5 and 6 contain rat and mouse brain tissue lysates. Reduced lysates containing 30 ug total protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection employed an HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate.