DNAJA3 Antibody / DnaJ homolog subfamily A member 3, mitochondrial Antibody (FY13576)

DNAJA3 Antibody by IF in HeLa cells. Immunofluorescence using DnaJ homolog subfamily A member 3, mitochondrial Antibody reveals granular green cytoplasmic staining surrounding blue DAPI-stained nuclei. Red alpha-tubulin counterstaining outlines the cell bodies and cytoskeletal network, while the green signal is enriched in discrete cytoplasmic structures. DNAJA3 is known to associate with mitochondria, although this image does not include a mitochondrial colocalization marker. Following enzyme antigen retrieval and goat serum blocking, cells were incubated with 5 ug/mL primary antibody overnight at 4oC and visualized using fluorescent secondary antibodies.

DNAJA3 Antibody by WB in human cell lines. Western blot analysis using DnaJ homolog subfamily A member 3, mitochondrial Antibody detects a band near 40 kDa in HeLa (lane 1), K562 (lane 2), MCF-7 (lane 3), and A549 (lane 4), compared with a calculated molecular weight of approximately 52 kDa. Published studies document processed mitochondrial TID1 isoforms of approximately 40 and 43 kDa, although the detected band has not been confirmed as a specific isoform. Reduced lysates containing 30 ug total protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk, followed by primary antibody incubation at 0.5 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and chemiluminescent substrate.