DNAJA1 Antibody / HSP40 HDJ2 Polyclonal Antibody (F54837)

DNAJA1 Antibody by IHC in human prostate carcinoma. Immunohistochemistry of FFPE human prostate carcinoma tissue using DNAJA1 Antibody. Brown DAB immunoreactivity is visible in tumor cells, with predominantly cytoplasmic staining and variable intensity among cells. Hematoxylin counterstaining highlights blue nuclei and surrounding tissue architecture. The staining pattern demonstrates HDJ2 immunoreactivity in malignant epithelial cells. This HSP40 HDJ2 Polyclonal Antibody recognizes a molecular co-chaperone involved in HSP70-mediated protein folding and cellular stress responses.

DNAJA1 Antibody by WB in MCF7 cells. Western blot analysis of human MCF7 cell lysate using DNAJA1 Antibody. A distinct immunoreactive band appears at approximately 45 kDa, consistent with the predicted molecular weight of DNAJA1. The band is relatively sharp, with minimal additional background staining in the displayed molecular-weight range. This HSP40 HDJ2 Polyclonal Antibody detects the endogenous co-chaperone also known as HDJ2, which participates in HSP70-dependent protein folding and cellular proteostasis.

DNAJA1 Antibody by WB in DNAJA1-transfected 293 cells. Western blot analysis comparing non-transfected (-) and DNAJA1-transfected (+) human 293 cells using DNAJA1 Antibody. A prominent band near 55 kDa is detected in the transfected sample but not in the negative control. This band migrates approximately 10 kDa above the expected 45 kDa molecular weight of endogenous DNAJA1. The difference may reflect a tagged expression construct or altered migration, although the cause is not established. This HSP40 HDJ2 Polyclonal Antibody recognizes a transfection-associated protein under the tested conditions.

DNAJA1 Antibody by FACS in MCF7 cells. Flow cytometric analysis of human MCF7 cells using DNAJA1 Antibody. The antibody-stained population exhibits increased fluorescence relative to the negative control, indicating antibody-associated detection of DNAJA1. This HSP40 HDJ2 Polyclonal Antibody recognizes HDJ2, a molecular co-chaperone involved in cellular protein quality control. Because DNAJA1 is primarily intracellular, the fluorescence shift should not be interpreted as evidence of cell-surface expression without confirmation of the staining and permeabilization conditions.