DNAAF2 Antibody / Protein kintoun Antibody (FY13647)

DNAAF2 Antibody by IHC in human thyroid cancer tissue. Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using Protein kintoun Antibody. Brown DAB immunoreactivity is visible in tumor-associated cellular structures, with blue hematoxylin counterstaining. The section underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocking with 10% goat serum. Primary antibody was applied at 2 ug/ml overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG.

DNAAF2 Antibody by IF in cultured human cells. Immunofluorescence using Protein kintoun Antibody shows green signal prominently within nuclei and a red alpha tubulin counterstain across the cytoplasm. The composite image includes blue nuclear staining. Fluorescent secondary antibodies distinguish the rabbit primary antibody from the mouse alpha tubulin antibody; the image demonstrates the distribution of immunoreactivity in the tested cells without establishing organelle identity.

DNAAF2 Antibody by WB in human, rat and mouse lysates. Western blot analysis using Protein kintoun Antibody with reducing lysates separated on a 10% SDS-PAGE gel. The five sample lanes are PC-3 human cells, RT4 human cells, rat ovary, rat testis and mouse testis, respectively. The dominant immunoreactive band migrates near 91 kDa across the samples, consistent with the predicted molecular weight of DNAAF2. Additional lower bands are visible in some lanes but are not assigned to a particular protein species. The blot was developed using an HRP-conjugated secondary antibody and chemiluminescent detection.