- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
DNA nucleotidylexotransferase Antibody / TdT [clone DNTT/4506R] (V8879)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V8879-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V8879-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V8879SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | DNTT/4506R |
| Purity | Protein A/G affinity |
| UniProt | P04053 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This DNA nucleotidylexotransferase antibody is available for research use only. |
|
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DNA nucleotidylexotransferase antibody, also known as Terminal deoxynucleotidyl transferase antibody, recognizes a specialized nuclear DNA polymerase encoded by the DNTT gene on chromosome 10q23-q24. DNA nucleotidylexotransferase Antibody (clone DNTT/4506R) detects Terminal deoxynucleotidyl transferase, commonly referred to as TdT, a member of the DNA polymerase X family selectively expressed in immature lymphoid cells. DNA nucleotidylexotransferase antibody, also referred to as DNTT antibody and TdT antibody in the literature, is widely used in research focused on lymphoid differentiation and hematologic malignancies.
Terminal deoxynucleotidyl transferase catalyzes the template-independent addition of deoxynucleotides to the 3-prime hydroxyl termini of DNA during V(D)J recombination. This unique enzymatic activity introduces N-region nucleotide diversity within immunoglobulin and T cell receptor gene segments, significantly expanding antigen receptor repertoire complexity. TdT functions within nuclear recombination centers in coordination with RAG1 and RAG2 complexes and other components of the non-homologous end joining pathway. Its catalytic core contains conserved polymerase domains characteristic of the Pol X family, enabling nucleotide incorporation without requiring a DNA template. A DNA nucleotidylexotransferase antibody therefore supports studies of adaptive immune system development and antigen receptor assembly.
DNTT expression is tightly regulated and largely restricted to early B and T lymphoblasts in bone marrow and thymus. In thymic cortex, TdT-positive cells represent developing T cell precursors undergoing receptor rearrangement. Expression decreases as lymphocytes mature, making TdT a well-established marker of lymphoid immaturity. Elevated nuclear TdT expression is frequently observed in acute lymphoblastic leukemia and lymphoblastic lymphoma, where DNA nucleotidylexotransferase antibody detection supports investigation of leukemic blast populations and lymphoid neoplasia biology.
Structurally, TdT contains regulatory regions that influence substrate selection and protein-protein interactions within recombination complexes. Alternative splicing of DNTT can generate isoforms with subtle biochemical differences that may affect catalytic efficiency and regulation. Persistent or dysregulated expression of DNTT contributes to genomic variability and is implicated in lymphoid malignancy development. Through its central role in immune receptor diversification, Terminal deoxynucleotidyl transferase remains essential to both normal lymphopoiesis and disease-oriented research applications.
Optimal dilution of the DNA nucleotidylexotransferase antibody should be determined by the researcher.
A portion of amino acids 1-100 from the human protein was used as the immunogen for the DNA nucleotidylexotransferase antibody.
Aliquot the DNA nucleotidylexotransferase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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